J Phys Act Health. 2021 Apr 12;18(6):653-659. doi: 10.1123/jpah.2020-0761. Print 2021 Jun 1.
Benefits of regular physical exercise were demonstrated as preventive and coadjuvant nonpharmacological anticancer therapy. However, the role of exercise in modulating prostate cancer behavior has yet to be established.
Prostate tumors were induced in C57BL/6 male mice (n = 28) by subcutaneous inoculation of a suspension of murine androgen-independent RM1 cells (1.5 × 105 cells/500 μL phosphate-buffered saline) in the dorsal region. Mice were randomly allocated into 2 study groups: sedentary tumor-induced (n = 14) and exercised tumor-induced (n = 14). Exercise consisted of voluntary running in wheeled cages. Mice (n = 7 per group) were sacrificed either 14 or 28 days after cell inoculation to evaluate tumor weight and percentage of area occupied by immunohistochemistry stained cells for Ki-67 and TdT-mediated dUTP-biotin nick end labeling, used as surrogate markers of cell proliferation and apoptosis, respectively.
Compared with sedentary tumor-induced mice, the tumors developed by exercised tumor-induced mice were significantly smaller at 14 days (0.17 [0.12] g vs 0.48 [0.24] g, P < .05) and at 28 days (0.92 [0.73] g vs 2.09 [1.31] g, P < .05), with smaller Ki-67 and greater TdT-mediated dUTP-biotin nick end-labeling stained areas (P < .05).
These results suggest that regular voluntary running inhibits prostate cancer cell growth by reducing cell proliferation and enhancing apoptosis.
定期进行体育锻炼的益处已被证明是一种预防和辅助的非药物抗癌疗法。然而，运动在调节前列腺癌行为中的作用尚未确定。
通过在背部皮下接种悬浮的鼠雄激素非依赖性 RM1 细胞（1.5×105 个细胞/500μL 磷酸盐缓冲液），在 C57BL/6 雄性小鼠（n=28）中诱导前列腺肿瘤。将小鼠随机分为 2 个研究组：久坐肿瘤诱导组（n=14）和运动肿瘤诱导组（n=14）。运动包括在轮式笼子中自愿跑步。每组（n=7）分别在细胞接种后 14 或 28 天处死，以评估肿瘤重量和用 Ki-67 和 TdT 介导的 dUTP-生物素切口末端标记法染色的细胞百分比，分别作为细胞增殖和细胞凋亡的替代标志物。
与久坐肿瘤诱导的小鼠相比，运动肿瘤诱导的小鼠的肿瘤在 14 天时明显更小（0.17[0.12]g 与 0.48[0.24]g，P<.05）和 28 天时（0.92[0.73]g 与 2.09[1.31]g，P<.05），Ki-67 染色面积较小，TdT 介导的 dUTP-生物素切口末端标记法染色面积较大（P<.05）。
这些结果表明，规律的自愿跑步通过减少细胞增殖和增强细胞凋亡来抑制前列腺癌细胞的生长。
