Zalkhani Raha, Moazedi Ahmad Ali, Ghotbeddin Zohreh, Pourmahdi Mahdi
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Basic Clin Neurosci. 2020 Nov-Dec;11(6):831-839. doi: 10.32598/bcn.11.6.1392.2. Epub 2020 Nov 1.
The interaction between antiepileptic drugs and brain electrical stimulation is a potential therapy to control seizures in patients with pharmacoresistance to drugs. So, the present study aimed to design to determine the effect of a subeffective dose of sodium valproate combined with low-frequency electrical stimulation during kindling.
One tripolar electrode was implanted stereotactically in the CA1 hippocampus of male Wistar rats. One week after surgery, the rats were kindled by electrical stimulation of hippocampus in a rapid manner (12 stimulations/day) for 6 days with sodium valproate alone or combined with low-frequency electrical stimulation (four packages contained 200 monophasic square wave pulses of 0.1-ms duration at 1 Hz, immediately after kindling stimulations). The duration of afterdischarge, maximum latency to stages 4 and 5, and the maximum duration of these stages were recorded by electromadule during kindling.
Application of sodium valproate with low-frequency electrical stimulation caused a reduction in cumulative afterdischarge duration. The maximum latency to the onset of stage 5 seizure increased after sodium valproate application alone, without having a significant effect on the fourth stage. Our findings showed reductions in the seizures duration and increasing in the latency times of both stages after the application of sodium valproate with low-frequency electrical stimulation.
It seems that usage of sodium valproate with low-frequency electrical stimulation during kindling was more effective to suppress the epileptic activity than its administration alone and may have a critical role on the antiepileptic effects of sodium valproate.
抗癫痫药物与脑电刺激之间的相互作用是一种控制药物耐药性患者癫痫发作的潜在疗法。因此，本研究旨在设计确定亚有效剂量的丙戊酸钠联合低频电刺激在点燃过程中的作用。
将一个三极电极立体定向植入雄性Wistar大鼠的海马CA1区。术后一周，大鼠通过海马电刺激快速点燃（每天12次刺激）6天，单独使用丙戊酸钠或联合低频电刺激（在点燃刺激后立即给予，四个包，包含200个持续时间为0.1毫秒、频率为1赫兹的单相方波脉冲）。在点燃过程中，通过电模块记录放电后持续时间、达到4期和5期的最大潜伏期以及这些阶段的最大持续时间。
丙戊酸钠联合低频电刺激应用导致累积放电后持续时间缩短。单独应用丙戊酸钠后，5期癫痫发作开始的最大潜伏期增加，而对4期无显著影响。我们的研究结果表明，丙戊酸钠联合低频电刺激应用后，癫痫发作持续时间缩短，两个阶段的潜伏期均延长。
似乎在点燃过程中使用丙戊酸钠联合低频电刺激比单独给药更有效地抑制癫痫活动，并且可能对丙戊酸钠的抗癫痫作用起关键作用。
