关于四硼酸钠作为肥厚性细胞内信号调节因子的数据。
Data on sodium tetraborate as a modulation of hypertrophic intracellular signals.
作者信息
Roque-Jorge J, Hernández-Gutiérrez S, Díaz-Rosas G, García-Chequer A J, López-Torres A, Contreras-Ramos A
机构信息
Univerisidad de Papaloapan, Oaxaca, México.
School of Medicine, Universidad Panamericana, Mexico City, Mexico.
出版信息
Data Brief. 2021 Feb 18;35:106889. doi: 10.1016/j.dib.2021.106889. eCollection 2021 Apr.
The present work benefits the use of sodium tetraborate to prevent and treat hypertrophic cardiac. The data obtained from the work could serve as a reference point to compare with data obtained in vivo studies with cardiac damage. This research will be an advantage for future researches to stimulate the ones focused on developing food supplements to prevent heart diseases such as cardiac hypertrophic. This article also indicates the data on the optimal concentration of isoproterenol as an inducer of hypertrophy in cardiomyocytes. Also, data of the cytotoxic effect of sodium tetraborate on normal cardiomyocytes is revealed. Finally, data of viability, cell size, proliferation nuclear antigen (PCNA) and apoptosis is shown. The expression of transcription factors linked to hypertrophy such as GATA-4, MEF2c, NFAT, CDk9, and myogenin was also quantified by immunofluorescence. The mRNA expression of adrenergic receptors (alpha and beta), AKT1 and Erk1 / 2 and genes of early response to hypertrophy () are also shown as Cts of RT-qPCR. GAPDH and 18 s were used as housekeeping genes.
目前的工作得益于使用四硼酸钠来预防和治疗肥厚性心肌病。从这项工作中获得的数据可以作为一个参考点，与在心脏损伤的体内研究中获得的数据进行比较。这项研究将有利于未来的研究，激励那些专注于开发预防心脏病（如心肌肥厚）的食品补充剂的研究。本文还指出了异丙肾上腺素作为心肌细胞肥大诱导剂的最佳浓度数据。此外，还揭示了四硼酸钠对正常心肌细胞的细胞毒性作用数据。最后，展示了细胞活力、细胞大小、增殖细胞核抗原（PCNA）和细胞凋亡的数据。与肥大相关的转录因子如GATA-4、MEF2c、NFAT、CDk9和肌细胞生成素的表达也通过免疫荧光进行了定量。肾上腺素能受体（α和β）、AKT1和Erk1 / 2的mRNA表达以及肥大早期反应基因（）也作为RT-qPCR的Cts显示出来。GAPDH和18 s用作看家基因。
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