Institute of Child Health, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan; Department of Pharmacy Technician Studies, Bayelsa State College of Health Technology, Ibadan, Nigeria.
Niger J Clin Pract. 2021 Apr;24(4):496-504. doi: 10.4103/njcp.njcp_491_20.
Malaria rapid diagnostic tests (mRDTs) are the preferred option for programmatic deployment.
There are numerous mRDTs on the Nigerian market and there is a need to guide practitioners on the relative performance of the commonly used brands of mRDT in Nigeria.
The performance of three commonly used Histidine-Rich-Protein-2-based mRDTs (SD-Bioline™, Carestart™ and Paracheck-Pf™) against microscopy of Giemsa stained blood and polymerase chain reaction (PCR) was evaluated among 190 febrile under-5 children in Ibadan, Nigeria. We calculated the sensitivity, specificity, predictive values, accuracy, and agreements.
There were 53.2% males. The prevalence of malaria parasite by microscopy was 46.8% and 57.9% by PCR. Malaria parasite detection by SD-Bioline™ was 60.5%, Carestart™: 60.0% and Paracheck-Pf™ 60.0%. Using microscopy as the gold standard, the sensitivities of SD-Bioline™, Carestart™ and Paracheck-Pf™ mRDT were 97.8%, 96.7% and 97.8% respectively while the specificities were 73.0%, 72.0% and 74.0% respectively. Using PCR as the gold standard, the sensitivity for both SD-Bioline™ and Paracheck-Pf™ was 85.5% and for CareStart was 84.6% while the specificity of SD-Bioline™, Carestart™, and Paracheck-Pf™ was 73.8%, 72.4%, and 75.0% respectively. The test accuracy was 81.0% for both SD-Bioline™ and Paracheck-Pf™ and 80.0% for Caresatrt™. The kappa coefficient of agreement between PCR and each of SD-Bioline™, Carestart, ParaCheck™ and microscopy was 0.597, 0.578, 0.609 and 0.739 respectively.
The performance of the three mRDTs is a proof that any of the three is suitable for use in the diagnosis of malaria in the southwest of Nigeria.
疟疾快速诊断检测（mRDT）是方案部署的首选方案。
尼日利亚市场上有许多 mRDT，有必要指导从业者了解尼日利亚常用 mRDT 品牌的相对性能。
在尼日利亚伊巴丹，对 190 名发热 5 岁以下儿童进行了三种常用组氨酸丰富蛋白 2 基 mRDT（SD-Bioline™、Carestart™和 Paracheck-Pf™）与吉姆萨染色血镜检和聚合酶链反应（PCR）的比较评估。我们计算了敏感性、特异性、预测值、准确性和一致性。
男性占 53.2%。镜检疟原虫的患病率为 46.8%，PCR 为 57.9%。SD-Bioline™检测疟原虫的比例为 60.5%，Carestart™：60.0%，Paracheck-Pf™：60.0%。以镜检为金标准，SD-Bioline™、Carestart™和 Paracheck-Pf™ mRDT 的敏感性分别为 97.8%、96.7%和 97.8%，特异性分别为 73.0%、72.0%和 74.0%。以 PCR 为金标准，SD-Bioline™和 Paracheck-Pf™的检测灵敏度均为 85.5%，CareStart 为 84.6%，而 SD-Bioline™、Carestart™和 Paracheck-Pf™的特异性分别为 73.8%、72.4%和 75.0%。SD-Bioline™和 Paracheck-Pf™的检测准确率均为 81.0%，CareStart™为 80.0%。PCR 与 SD-Bioline™、Carestart、ParaCheck™和显微镜之间的kappa 一致性系数分别为 0.597、0.578、0.609 和 0.739。
三种 mRDT 的性能证明，在尼日利亚西南部诊断疟疾时，任何一种 mRDT 都适用。
