塞内加尔宫颈病变中高危型 HPV 基因型的各自流行率。

Respective prevalence of high-risk HPVgenotypes in cervical neoplasia in Senegal.

机构信息

Bacteriology-Virology Laboratory at CHU Aristide Le Dantec, Aristide Le Dantec Hospital, Cheikh Anta Diop University of Dakar, Dakar, Sénégal.

Department of Pathology, Intercommunal Hospital Center of Créteil, Créteil Cedex, France.

出版信息

J Med Virol. 2021 Aug;93(8):5110-5117. doi: 10.1002/jmv.27020. Epub 2021 Apr 23.

DOI:10.1002/jmv.27020

PMID:33851737

Abstract

OBJECTIVES

With the perspective of prophylactic vaccination against high-risk human papillomavirus (HPV), we analyzed the viral epidemiology of cervical neoplasia in Senegal.

METHODS

All patients were treated at the Institut Joliot Curie du Cancer in Dakar. HPV genotypes were characterized using a real-time polymerase chain reaction-based approach and sequencing.

RESULTS

Histologically, there were 224 invasive carcinomas, 17 high-grade intraepithelial neoplasia (CIN), and five undetermined histologies. Molecular analysis was conclusive in 241 cases. HPV DNA was found in 207/241 (85.9%) cases while 34/241 (14.1%) remained HPV negative. There was one single genotype in 127/207 (61.4%) cases and several in 80/207 (38.6%) corresponding to 308 genotypes identified. Viral genotyping found HPV16 in 175 (56.8%) cases, HPV18 in 45 (14.6%), HPV45 in 40 (13.0%), HPV58 in 35 (11.4%), HPV33 in 6 (2.0%), HPV35 in 3 (1.0%), HPV31 in 2 (0.6%), HPV39 and HPV56 in one (0.3% each).

CONCLUSION

Our analysis showed that 98.4% of the HPV-positive cases were associated with viral genotypes covered by the 9-valent HPV vaccine. However, 14.1% of cases remained HPV negative. Therefore, prophylactic vaccination using a 9-valent vaccine should dramatically reduce the incidence of HPV-associated neoplasia but the detection and treatment of CIN remain necessary for the optimal prevention of cervical cancer.

摘要

目的

从预防性接种高危型人乳头瘤病毒（HPV）疫苗的角度出发，我们分析了塞内加尔宫颈癌的病毒流行病学。

方法

所有患者均在达喀尔的居里研究所接受治疗。采用实时聚合酶链反应（PCR）方法和测序对 HPV 基因型进行特征分析。

结果

组织学上，有 224 例浸润性癌、17 例高级别上皮内瘤变（CIN）和 5 例未确定的组织学类型。241 例进行了分子分析。207/241（85.9%）例 HPV DNA 阳性，34/241（14.1%）例 HPV DNA 阴性。127/207（61.4%）例为单一基因型，80/207（38.6%）例为多种基因型，共鉴定出 308 种基因型。病毒基因分型发现 HPV16 感染 175 例（56.8%），HPV18 感染 45 例（14.6%），HPV45 感染 40 例（13.0%），HPV58 感染 35 例（11.4%），HPV33 感染 6 例（2.0%），HPV35 感染 3 例（1.0%），HPV31 感染 2 例（0.6%），HPV39 和 HPV56 各感染 1 例（0.3%）。