• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

细胞内穆斯堡尔和电子顺磁共振研究为人抗肌萎缩蛋白中铁硫簇结合提供新证据

In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long-Standing Debate on Iron-Sulfur Cluster Binding in Human Anamorsin.

机构信息

Magnetic Resonance Center CERM, University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy.

Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine (CIRMMP), Via L. Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy.

出版信息

Angew Chem Int Ed Engl. 2021 Jun 25;60(27):14841-14845. doi: 10.1002/anie.202102910. Epub 2021 May 24.

DOI:10.1002/anie.202102910
PMID:33852169
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8251831/
Abstract

Human anamorsin is an iron-sulfur (Fe-S)-cluster-binding protein acting as an electron donor in the early steps of cytosolic iron-sulfur protein biogenesis. Human anamorsin belongs to the eukaryotic CIAPIN1 protein family and contains two highly conserved cysteine-rich motifs, each binding an Fe-S cluster. In vitro works by various groups have provided rather controversial results for the type of Fe-S clusters bound to the CIAPIN1 proteins. In order to unravel the knot on this topic, we used an in cellulo approach combining Mössbauer and EPR spectroscopies to characterize the iron-sulfur-cluster-bound form of human anamorsin. We found that the protein binds two [2Fe-2S] clusters at both its cysteine-rich motifs.

摘要

人源 anamorsin 是一种铁硫簇（Fe-S）结合蛋白，在细胞质铁硫蛋白生物发生的早期步骤中作为电子供体发挥作用。人源 anamorsin 属于真核 CIAPIN1 蛋白家族，含有两个高度保守的富含半胱氨酸的基序，每个基序都结合一个 Fe-S 簇。不同研究小组的体外研究结果对结合到 CIAPIN1 蛋白上的 Fe-S 簇的类型提供了相当有争议的结果。为了解开这个谜团，我们使用体内方法结合 Mössbauer 和 EPR 光谱学来表征人源 anamorsin 的铁硫簇结合形式。我们发现该蛋白在其两个富含半胱氨酸的基序处均结合两个 [2Fe-2S] 簇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/8251831/67c9e8d4d968/ANIE-60-14841-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/8251831/96aa03383bf6/ANIE-60-14841-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/8251831/67c9e8d4d968/ANIE-60-14841-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/8251831/96aa03383bf6/ANIE-60-14841-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/8251831/67c9e8d4d968/ANIE-60-14841-g002.jpg

相似文献

1
In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long-Standing Debate on Iron-Sulfur Cluster Binding in Human Anamorsin.细胞内穆斯堡尔和电子顺磁共振研究为人抗肌萎缩蛋白中铁硫簇结合提供新证据
Angew Chem Int Ed Engl. 2021 Jun 25;60(27):14841-14845. doi: 10.1002/anie.202102910. Epub 2021 May 24.
2
Human anamorsin binds [2Fe-2S] clusters with unique electronic properties.人类anamorsin与具有独特电子性质的[2Fe-2S]簇结合。
J Biol Inorg Chem. 2013 Dec;18(8):883-93. doi: 10.1007/s00775-013-1033-1. Epub 2013 Aug 30.
3
Cancer-Related NEET Proteins Transfer 2Fe-2S Clusters to Anamorsin, a Protein Required for Cytosolic Iron-Sulfur Cluster Biogenesis.癌症相关的NEET蛋白将2Fe-2S簇转移至Anamorsin，后者是胞质铁硫簇生物合成所需的一种蛋白质。
PLoS One. 2015 Oct 8;10(10):e0139699. doi: 10.1371/journal.pone.0139699. eCollection 2015.
4
The conserved protein Dre2 uses essential [2Fe-2S] and [4Fe-4S] clusters for its function in cytosolic iron-sulfur protein assembly.保守蛋白Dre2在胞质铁硫蛋白组装中发挥功能时，利用必需的[2Fe-2S]和[4Fe-4S]簇。
Biochem J. 2016 Jul 15;473(14):2073-85. doi: 10.1042/BCJ20160416. Epub 2016 May 10.
5
Anamorsin is a [2Fe-2S] cluster-containing substrate of the Mia40-dependent mitochondrial protein trapping machinery.Anamorsin是一种依赖于Mia40的线粒体蛋白质捕获机制的含[2Fe-2S]簇的底物。
Chem Biol. 2011 Jun 24;18(6):794-804. doi: 10.1016/j.chembiol.2011.03.015.
6
Anamorsin/Ndor1 Complex Reduces [2Fe-2S]-MitoNEET via a Transient Protein-Protein Interaction.Anamorsin/Ndor1 复合物通过瞬时蛋白-蛋白相互作用来减少 [2Fe-2S]-MitoNEET。
J Am Chem Soc. 2017 Jul 19;139(28):9479-9482. doi: 10.1021/jacs.7b05003. Epub 2017 Jul 6.
7
N-terminal domains mediate [2Fe-2S] cluster transfer from glutaredoxin-3 to anamorsin.N 端结构域介导[2Fe-2S]簇从谷氧还蛋白 3 到 anamorsin 的转移。
Nat Chem Biol. 2015 Oct;11(10):772-8. doi: 10.1038/nchembio.1892. Epub 2015 Aug 24.
8
EPR studies of wild type and mutant Dre2 identify essential [2Fe--2S] and [4Fe--4S] clusters and their cysteine ligands.野生型和突变型Dre2的电子顺磁共振研究确定了必需的[2铁-2硫]和[4铁-4硫]簇及其半胱氨酸配体。
J Biochem. 2017 Jan;161(1):67-78. doi: 10.1093/jb/mvw054. Epub 2016 Sep 26.
9
Biochemical Reconstitution and Spectroscopic Analysis of Iron-Sulfur Proteins.铁硫蛋白的生化重建与光谱分析
Methods Enzymol. 2018;599:197-226. doi: 10.1016/bs.mie.2017.11.034. Epub 2018 Jan 10.
10
Unraveling the mechanism of [4Fe-4S] cluster assembly on the N-terminal cluster binding site of NUBP1.阐明 NUBP1 N 端簇结合位点上 [4Fe-4S] 簇组装的机制。
Protein Sci. 2023 May;32(5):e4625. doi: 10.1002/pro.4625.

引用本文的文献

1
Requirements for the biogenesis of [2Fe-2S] proteins in the human and yeast cytosol.人类和酵母细胞质中 [2Fe-2S] 蛋白生物发生的要求。
Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2400740121. doi: 10.1073/pnas.2400740121. Epub 2024 May 14.
2
Unraveling the mechanism of [4Fe-4S] cluster assembly on the N-terminal cluster binding site of NUBP1.阐明 NUBP1 N 端簇结合位点上 [4Fe-4S] 簇组装的机制。
Protein Sci. 2023 May;32(5):e4625. doi: 10.1002/pro.4625.
3
Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei.

本文引用的文献

1
Apd1 and Aim32 Are Prototypes of Bishistidinyl-Coordinated Non-Rieske [2Fe-2S] Proteins.Apd1 和 Aim32 是双组氨酸配位的非 Rieske [2Fe-2S] 蛋白的原型。
J Am Chem Soc. 2019 Apr 10;141(14):5753-5765. doi: 10.1021/jacs.8b13274. Epub 2019 Mar 26.
2
EPR spectroscopy of complex biological iron-sulfur systems.复杂生物铁硫体系的电子顺磁共振波谱学。
J Biol Inorg Chem. 2018 Jun;23(4):623-634. doi: 10.1007/s00775-018-1543-y. Epub 2018 Feb 21.
3
Protein networks in the maturation of human iron-sulfur proteins.人类铁硫蛋白成熟过程中的蛋白质网络。
基于穆斯堡尔谱的酿酒酵母全铁组学分子水平解析，以及分离核的初步特征描述。
Metallomics. 2022 Nov 1;14(11). doi: 10.1093/mtomcs/mfac080.
Metallomics. 2018 Jan 24;10(1):49-72. doi: 10.1039/c7mt00269f.
4
ISCA1 is essential for mitochondrial FeS biogenesis in vivo.ISCA1 对于体内线粒体 Fe-S 生物发生是必需的。
Nat Commun. 2017 May 11;8:15124. doi: 10.1038/ncomms15124.
5
NBP35 interacts with DRE2 in the maturation of cytosolic iron-sulphur proteins in Arabidopsis thaliana.在拟南芥胞质铁硫蛋白成熟过程中，NBP35与DRE2相互作用。
Plant J. 2017 Feb;89(3):590-600. doi: 10.1111/tpj.13409. Epub 2017 Feb 3.
6
EPR studies of wild type and mutant Dre2 identify essential [2Fe--2S] and [4Fe--4S] clusters and their cysteine ligands.野生型和突变型Dre2的电子顺磁共振研究确定了必需的[2铁-2硫]和[4铁-4硫]簇及其半胱氨酸配体。
J Biochem. 2017 Jan;161(1):67-78. doi: 10.1093/jb/mvw054. Epub 2016 Sep 26.
7
The conserved protein Dre2 uses essential [2Fe-2S] and [4Fe-4S] clusters for its function in cytosolic iron-sulfur protein assembly.保守蛋白Dre2在胞质铁硫蛋白组装中发挥功能时，利用必需的[2Fe-2S]和[4Fe-4S]簇。
Biochem J. 2016 Jul 15;473(14):2073-85. doi: 10.1042/BCJ20160416. Epub 2016 May 10.
8
The role of mitochondria and the CIA machinery in the maturation of cytosolic and nuclear iron-sulfur proteins.线粒体和 CIA 机器在细胞质和核铁硫蛋白成熟中的作用。
Eur J Cell Biol. 2015 Jul-Sep;94(7-9):280-91. doi: 10.1016/j.ejcb.2015.05.002. Epub 2015 May 31.
9
Cytosolic iron-sulphur protein assembly is functionally conserved and essential in procyclic and bloodstream Trypanosoma brucei.胞质铁硫蛋白组装在原循环型和血流型布氏锥虫中功能保守且至关重要。
Mol Microbiol. 2014 Sep;93(5):897-910. doi: 10.1111/mmi.12706. Epub 2014 Jul 23.
10
Crystal structure of the N-terminal methyltransferase-like domain of anamorsin.阿诺莫辛N端类甲基转移酶结构域的晶体结构
Proteins. 2014 Jun;82(6):1066-71. doi: 10.1002/prot.24443. Epub 2013 Nov 22.