Shin Kyung-Chul, Kil Tae-Geun, Lee Tae-Eui, Oh Deok-Kun
Department of Integrative Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
J Agric Food Chem. 2021 Apr 28;69(16):4766-4777. doi: 10.1021/acs.jafc.0c06756. Epub 2021 Apr 14.
Extract from balloon flower root (Platycodi radix) containing platycosides as saponins is a beneficial food additive and is used for their savory taste and the alleviation of respiratory diseases. Deglycosylated platycosides show greater pharmacological effects than glycosylated platycosides. However, there are no reports on the conversion of glycosylated platycosides into deapiosylated platycosides. In this study, we showed that the crude enzyme from , a generally recognized as safe (GRAS) fungus isolated from meju (fermented soybean brick), completely converted glycosylated platycosides in Platycodi radix extract into deapiosylated platycosides: deapiosylated platycodin D (deapi-PD), deapiosylated platycodin A (deapi-PA), deapiosylated polygalacin D (deapi-PGD), and deapiosylated platyconic acid A (deapi-PCA). Among these, deapi-PA and deapi-PCA were first identified using liquid chromatography/mass spectrometry. The anti-inflammatory and antioxidant effects of deapiosylated platycosides were greater than those of the precursor glycosylated platycosides. These deapiosylated platycosides could improve the properties of functional food additives.
桔梗根提取物（桔梗根）含有作为皂苷的桔梗皂苷，是一种有益的食品添加剂，因其美味的口感和对呼吸道疾病的缓解作用而被使用。去糖基化的桔梗皂苷比糖基化的桔梗皂苷显示出更大的药理作用。然而，关于糖基化桔梗皂苷转化为去芹菜糖基化桔梗皂苷的报道尚未见。在本研究中，我们发现从梅久（发酵大豆砖）中分离出的一种公认为安全（GRAS）的真菌的粗酶，能将桔梗根提取物中的糖基化桔梗皂苷完全转化为去芹菜糖基化桔梗皂苷：去芹菜糖基化桔梗皂苷D（deapi-PD）、去芹菜糖基化桔梗皂苷A（deapi-PA）、去芹菜糖基化远志酸D（deapi-PGD）和去芹菜糖基化桔梗酸A（deapi-PCA）。其中，deapi-PA和deapi-PCA首次通过液相色谱/质谱法鉴定。去芹菜糖基化桔梗皂苷的抗炎和抗氧化作用大于前体糖基化桔梗皂苷。这些去芹菜糖基化桔梗皂苷可以改善功能性食品添加剂的性能。
