Pedrosa Mariana, Ferreira Maria Teresa, E Batista de Carvalho Luís A, M Marques Maria Paula, Curate Francisco
Centre for Functional Ecology, Laboratory of Forensic Anthropology, Department of Life Sciences, Calçada Martim de Freitas, University of Coimbra, Coimbra, Portugal.
Department of Life Sciences, Calçada Martim de Freitas, University of Coimbra, Coimbra, Portugal.
Am J Phys Anthropol. 2021 Nov;176(3):434-444. doi: 10.1002/ajpa.24283. Epub 2021 Apr 14.
Even though much is known about bone mineral and matrix composition, studies about their relationship with several bone properties and its alterations related to bone diseases such as osteoporosis are practically non-existent in humans. Thus, the development of methods to understand the effects of bone properties at a microscopic level is paramount. This research aimed to evaluate whether Fourier transform infrared-attenuated total reflectance (FTIR-ATR) band intensity ratios correlate with femoral bone mass, bone mineral content (BMC) (total and femoral neck), bone mineral per unit area (BMD) (total, femoral neck, greater trochanter, intertrochanteric region, and Ward's area) and the area (total and femoral neck). A sample of femora from the 21st Century Identified Skeleton Collection (N = 78, 42 females and 36 males) was employed and BMC, BMD, and the femoral areas were acquired by DXA.
It was found that only females' BMD had a significant association with the femoral FTIR-ATR indices under study, whereas bone collagen (Am/P) and the content of carbonate Type A (API) in males correlated with the total proximal femur area of the regions of interest and the femoral neck area.
Men and women showed different changes related to their chemical composition in BMD, BMC, and probed area, most likely due to differences in structure and physiology, as well as mechanical strength in the proximal femoral sites where BMD was analyzed.
尽管人们对骨矿物质和基质组成已有很多了解，但关于它们与多种骨特性的关系以及与骨质疏松等骨疾病相关的改变在人类中的研究实际上并不存在。因此，开发在微观层面理解骨特性影响的方法至关重要。本研究旨在评估傅里叶变换红外衰减全反射（FTIR - ATR）谱带强度比是否与股骨骨量、骨矿物质含量（BMC）（总体和股骨颈）、单位面积骨矿物质含量（BMD）（总体、股骨颈、大转子、转子间区域和沃德氏区）以及面积（总体和股骨颈）相关。使用了来自21世纪鉴定骨骼收藏的股骨样本（N = 78，42名女性和36名男性），并通过双能X线吸收法（DXA）获取BMC、BMD和股骨面积。
发现只有女性的BMD与所研究的股骨FTIR - ATR指标有显著关联，而男性的骨胶原蛋白（Am/P）和A型碳酸盐含量（API）与感兴趣区域的股骨近端总面积和股骨颈面积相关。
男性和女性在BMD、BMC和探测区域的化学成分方面表现出不同变化，这很可能是由于结构和生理差异以及分析BMD的股骨近端部位的机械强度差异所致。
