Universidade Anhanguera de São Paulo, UNIAN, Programa de Pós-Graduação em Farmácia e Biotecnologia e Inovação em Saúde, Rua Raimundo Pereira de Magalhães, 3305, Pirituba, Zip Code: 05145-200 São Paulo, SP, Brazil.
Universidade Anhanguera de São Paulo, UNIAN, Programa de Pós-Graduação em Biotecnologia e Inovação em Saúde, Rua Raimundo Pereira de Magalhães, 3305, Pirituba, Zip Code: 05145-200 São Paulo, SP, Brazil.
J Neuroimmunol. 2021 Jun 15;355:577563. doi: 10.1016/j.jneuroim.2021.577563. Epub 2021 Apr 7.
The combination of genetic and epigenetic influences alters the development of complex diseases. Aberrant patterns of DNA methylation are associated with inflammation and clinical activity in MS. We evaluated the differences between global DNA methylation in lymphocytes and monocytes of patients with MS compared to healthy controls. Thirty-three patients with RRMS (PwRMS) and five healthy individuals were included. DNA was isolated from PBMCs by a phenol-chloroform method, and global methylation was analyzed by Imprint® Methylated DNA Quantification Kit. We observed a cell-type-specific DNA methylation pattern and showed that monocyte global DNA methylation was significantly affected by IFNβ treatment.
遗传和表观遗传因素的共同作用改变了复杂疾病的发展。DNA 甲基化模式的异常与 MS 中的炎症和临床活动有关。我们评估了 MS 患者与健康对照者相比，淋巴细胞和单核细胞中整体 DNA 甲基化的差异。共纳入 33 例 RRMS 患者（PwRMS）和 5 名健康个体。采用酚-氯仿法从 PBMC 中分离 DNA，并用 Imprint® Methylated DNA Quantification Kit 分析整体甲基化。我们观察到了一种细胞类型特异性的 DNA 甲基化模式，并表明单核细胞整体 DNA 甲基化受 IFNβ 治疗的显著影响。
