Kodera Chie, Just Jérémy, Da Rocha Martine, Larrieu Antoine, Riglet Lucie, Legrand Jonathan, Rozier Frédérique, Gaude Thierry, Fobis-Loisy Isabelle
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Inria, F-69342, Lyon, France.
Present Address: Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000, Versailles, France.
BMC Genomics. 2021 Apr 14;22(1):268. doi: 10.1186/s12864-021-07503-7.
Fertilization in flowering plants depends on the early contact and acceptance of pollen grains by the receptive papilla cells of the stigma. Deciphering the specific transcriptomic response of both pollen and stigmatic cells during their interaction constitutes an important challenge to better our understanding of this cell recognition event.
Here we describe a transcriptomic analysis based on single nucleotide polymorphisms (SNPs) present in two Arabidopsis thaliana accessions, one used as female and the other as male. This strategy allowed us to distinguish 80% of transcripts according to their parental origins. We also developed a tool which predicts male/female specific expression for genes without SNP. We report an unanticipated transcriptional activity triggered in stigma upon incompatible pollination and show that following compatible interaction, components of the pattern-triggered immunity (PTI) pathway are induced on the female side.
Our work unveils the molecular signatures of compatible and incompatible pollinations both at the male and female side. We provide invaluable resource and tools to identify potential new molecular players involved in pollen-stigma interaction.
开花植物的受精取决于柱头的感受性乳突细胞对花粉粒的早期接触和接受。解析花粉与柱头细胞在相互作用过程中特定的转录组反应，对于加深我们对这种细胞识别事件的理解构成了一项重大挑战。
在此，我们描述了一种基于两个拟南芥生态型中存在的单核苷酸多态性（SNP）的转录组分析方法，其中一个用作雌性，另一个用作雄性。这种策略使我们能够根据转录本的亲本来源区分80%的转录本。我们还开发了一种工具，可预测无SNP基因的雄性/雌性特异性表达。我们报告了在不亲和授粉时柱头中引发的意外转录活性，并表明在亲和相互作用后，模式触发免疫（PTI）途径的成分在雌性一方被诱导。
我们的工作揭示了雄性和雌性双方亲和与不亲和授粉的分子特征。我们提供了宝贵的资源和工具，以鉴定参与花粉-柱头相互作用的潜在新分子参与者。
