Leibniz Institute of Photonic Technology (IPHT), Jena, Germany.
InfectoGnostics Research Campus Jena, Jena, Germany.
Sci Rep. 2021 Apr 14;11(1):8128. doi: 10.1038/s41598-021-86273-4.
While many data on molecular epidemiology of MRSA are available for North America, Western Europe and Australia, much less is known on the distribution of MRSA clones elsewhere. Here, we describe a poorly known lineage from the Middle East, CC1153, to which several strains from humans and livestock belong. Isolates were characterised using DNA microarrays and one isolate from the United Arab Emirates was sequenced using Nanopore technology. CC1153 carries agr II and capsule type 5 genes. Enterotoxin genes are rarely present, but PVL is common. Associated spa types include t504, t903 and t13507. PVL-positive CC1153-MSSA were found in Egyptian cattle suffering from mastitis. It was also identified among humans with skin and soft tissue infections in Saudi Arabia, France and Germany. CC1153-MRSA were mainly observed in Arabian Gulf countries. Some isolates presented with a previously unknown SCCmec/SCCfus chimeric element in which a mec B complex was found together with the fusidic acid resistance gene fusC and accompanying genes including ccrA/B-1 recombinase genes. Other isolates carried SCCmec V elements that usually also included fusC. Distribution and emergence of CC1153-MRSA show the necessity of molecular characterization of MRSA that are resistant to fusidic acid. These strains pose a public health threat as they combine resistance to beta-lactams used in hospitals as well as to fusidic acid used in the community. Because of the high prevalence of fusC-positive MRSA in the Middle East, sequences and descriptions of SCC elements harbouring fusC and/or mecA are reviewed. When comparing fusC and its surrounding regions from the CC1153 strain to available published sequences, it became obvious that there are four fusC alleles and five distinct types of fusC gene complexes reminiscent to the mec complexes in SCCmec elements. Likewise, they are associated with different sets of ccrA/B recombinase genes and additional payload that might include entire mec complexes or SCCmec elements.
虽然有许多关于北美、西欧和澳大利亚耐甲氧西林金黄色葡萄球菌（MRSA）分子流行病学的数据，但对其他地区 MRSA 克隆的分布知之甚少。在这里，我们描述了一个来自中东的鲜为人知的谱系 CC1153，其中包括一些来自人类和牲畜的菌株。使用 DNA 微阵列对分离株进行了特征描述，并且从阿拉伯联合酋长国分离出的一个分离株使用纳米孔技术进行了测序。CC1153 携带 agr II 和荚膜类型 5 基因。肠毒素基因很少存在，但 PVL 很常见。相关的 spa 类型包括 t504、t903 和 t13507。在患有乳腺炎的埃及牛中发现了 PVL 阳性的 CC1153-MSSA。在沙特阿拉伯、法国和德国患有皮肤和软组织感染的人群中也发现了这种情况。CC1153-MRSA 主要在阿拉伯海湾国家观察到。一些分离株具有一种以前未知的 SCCmec/SCCfus 嵌合元件，其中 mec B 复合物与 fusidic 酸抗性基因 fusC 以及包括 ccrA/B-1 重组酶基因在内的伴随基因一起发现。其他分离株携带 SCCmec V 元件，这些元件通常也包括 fusC。CC1153-MRSA 的分布和出现表明需要对耐 fusidic 酸的 MRSA 进行分子特征分析。这些菌株构成了公共卫生威胁，因为它们结合了对医院中使用的β-内酰胺类药物以及社区中使用的 fusidic 酸的耐药性。由于中东 fusC 阳性 MRSA 的高流行率，审查了携带 fusC 和/或 mecA 的 SCC 元件的序列和描述。将 CC1153 菌株的 fusC 及其周围区域与可用的已发表序列进行比较时，显然存在 4 种 fusC 等位基因和 5 种不同类型的 fusC 基因复合物，类似于 SCCmec 元件中的 mec 复合物。同样，它们与不同的 ccrA/B 重组酶基因和可能包括整个 mec 复合物或 SCCmec 元件的附加有效载荷相关联。
