Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430061, People's Republic of China.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430061, People's Republic of China.
J Agric Food Chem. 2021 Apr 28;69(16):4840-4848. doi: 10.1021/acs.jafc.0c07665. Epub 2021 Apr 15.
Biocontrol to combat the menace of has gained considerable attention. However, the molecular mechanisms of 's response to antagonism biotic stress are poorly deciphered. Here, we discovered that switches an adaptive metabolic reprogramming to ensure its adversity survival by multiomics analyses (including four omics platform). Antifungal "weapons" lipopeptides and antibacterial metabolites of imizoquin were identified. The central metabolism fluxes were significantly depleted but the expressions of most corresponding genes were considerably increased in . Secondary metabolism that does not contribute to stress was markedly suppressed. In contrast, antibacterial "weapon arsenal" was activated to occupy an ecological niche. Our results revealed that interlinked mitochondrial central metabolism and secondary metabolism are central to antagonism biotic stress response. This discovery contributes to the targeted design of biocontrol agents and smart regularization of rhizosphere microbiome homeostasis to realize long-term fungi pathogen control and mitigation mycotoxin contamination.
生物防治对抗 的威胁引起了相当大的关注。然而， 的抗生物胁迫的分子机制还未被完全破解。在这里，我们通过多组学分析（包括四个组学平台）发现， 切换到适应性代谢重编程，以确保其逆境生存。鉴定到了抗真菌的脂肽和抗菌代谢物 imizoquin。 中中央代谢通量显著减少，但大多数相应基因的表达显著增加。对非胁迫无贡献的次生代谢显著受到抑制。相反， 抗菌“武器库”被激活以占据生态位。我们的研究结果表明，相互关联的线粒体中央代谢和次生代谢是 对抗生物胁迫反应的关键。这一发现有助于有针对性地设计生物防治剂，并智能调节根际微生物组的内稳态，以实现长期真菌病原体控制和减轻真菌毒素污染。
