Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Changchun, 130021, Jilin, People's Republic of China.
Institute of Frontier Medical Science, Jilin University, No. 1163 Xinmin Street, Changchun, 130021, Jilin, People's Republic of China.
Protein Expr Purif. 2021 Aug;184:105888. doi: 10.1016/j.pep.2021.105888. Epub 2021 Apr 13.
Anti-EGFR nanobodies have been successfully applied as antitumor moieties in the photodynamic therapy and drug delivery systems. But the yields of nanobodies were still limited due to the volumetric capacity of the periplasmic compartments and inclusion bodies of Escherichia coli. A comparative study of Pichia pastoris and Escherichia coli was done through characterizing their products. Nanobody 7D12 and 7D12-9G8 were successfully expressed in Pichia pastoris with 6-13.6-fold higher yield. Both two types of nanobodies had internalization ability to be developed as antitumor moieties.
抗 EGFR 纳米抗体已成功应用于光动力治疗和药物传递系统中的抗肿瘤部分。但是，由于大肠杆菌周质腔和包涵体的体积容量限制，纳米抗体的产量仍然有限。通过对毕赤酵母和大肠杆菌进行比较研究，对它们的产物进行了表征。纳米抗体 7D12 和 7D12-9G8 在毕赤酵母中成功表达，产量提高了 6-13.6 倍。这两种类型的纳米抗体都具有内化能力，可作为抗肿瘤部分进行开发。
