Gorgas Memorial Institute of Health Studies, Panama, Panama; Coiba Scientific Station, Coiba AIP, City of Knowledge, Panama.
Gorgas Memorial Institute of Health Studies, Panama, Panama.
Ticks Tick Borne Dis. 2021 Jul;12(4):101723. doi: 10.1016/j.ttbdis.2021.101723. Epub 2021 Apr 6.
This paper presents new data about Rickettsia species detected in ticks collected from wild animals, using 16S rRNA, gltA and ompA. Rickettsia DNA was found in 66 of 101 ticks. Using EZ BioCloud libraries were produced reads that identified Rickettsia aeschlimannii, and Illumina BaseSpace produced reads of Rickettsia rickettsii group, Rickettsia bellii group, and unclassified Rickettsia. Using gltA and ompA gene-specific primers, R. aeschlimannii could not be confirmed, but detection of Rickettsia amblyommatis was achieved in Amblyomma auricularium, Amblyomma geayi, Amblyomma mixtum, and Amblyomma pacae; R. bellii from Amblyomma dissimile, "Candidatus Rickettsia colombianensi" from A. dissimile, Rickettsia spp. closely related to R. raoultii from A. geayi, Rickettsia tamurae from A. dissimile, and Rickettsia endosymbionts of Ixodes from Ixodes affinis. There were no databases available specifically for 16S rRNA of Neotropical Rickettsia, highlighting the need to use species primers over only 16S rRNA primers to achieve more accurate interpretations and identifications. These findings increase the number of Rickettsia species detected in Panama and highlight the need to establish isolates to further characterize the nature of Rickettsia in the area.
本文介绍了使用 16S rRNA、gltA 和 ompA 从野生动物采集的蜱中检测到的立克次体物种的新数据。在 101 只蜱中，有 66 只检测到立克次体 DNA。使用 EZ BioCloud 文库产生的读取序列可识别出 Rickettsia aeschlimannii，而 Illumina BaseSpace 产生的读取序列则鉴定为 Rickettsia rickettsii 组、Rickettsia bellii 组和未分类的立克次体。使用 gltA 和 ompA 基因特异性引物，无法确认 R. aeschlimannii，但在 Amblyomma auricularium、 Amblyomma geayi、Amblyomma mixtum 和 Amblyomma pacae 中检测到了 Rickettsia amblyommatis；在 Amblyomma dissimile 中检测到了 R. bellii，在 A. dissimile 中检测到了“Candidatus Rickettsia colombianensi”，在 A. geayi 中检测到了与 R. raoultii 密切相关的 Rickettsia spp.，在 A. dissimile 中检测到了 Rickettsia tamurae，在 Ixodes affinis 中检测到了 Ixodes 的内共生菌。目前没有专门针对 Neotropical Rickettsia 的 16S rRNA 数据库，这突出表明需要使用物种引物而不仅仅是 16S rRNA 引物，以实现更准确的解释和鉴定。这些发现增加了在巴拿马检测到的立克次体物种的数量，并强调需要建立分离株以进一步表征该地区立克次体的性质。
