Faculty of Food Technology and Biotechnology University of Zagreb, Department of Food Engineering, Pierottijeva 6, 10 000, Zagreb, Croatia.
INRAE, UR BIA Biopolymers - Interactions - Assemblies, F-44316, Nantes, France.
Carbohydr Polym. 2021 Jul 1;263:117932. doi: 10.1016/j.carbpol.2021.117932. Epub 2021 Mar 17.
According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus. The aim of this paper was to evaluate WS and PP as sources for the production of xylooligosaccharides (XOS). Hemicelluloses from WS and PP were recovered by combining varying parameters of delignification and alkaline extraction. At optimal recovery conditions, the fractions were further hydrolysed to XOS using GH11 endo-xylanase, by varying time and enzyme concentration. Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well. Delignification was essential for high recovery of total xylose from the materials, up to at least 70 %. High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.
鉴于人们对农业工业废物再利用的浓厚兴趣，利用率较低的木质纤维素材料，如核桃壳 (WS) 和豌豆荚 (PP)，成为关注焦点。本文旨在评估 WS 和 PP 作为生产木低聚糖 (XOS) 的原料。通过结合不同的脱木质素和碱性提取参数，从 WS 和 PP 中回收半纤维素。在最佳回收条件下，通过改变时间和酶浓度，使用 GH11 内切木聚糖酶将这些级分进一步水解为 XOS。在获得的半纤维素的单体组成中，木糖占主导地位，在 WS 中仅形成低支链（阿拉伯糖）木葡聚糖，而在 PP 中则存在一些木葡聚糖。木质素脱除对于从这些材料中高回收率的总木糖至关重要，至少达到 70%。24 小时水解可获得高木聚糖转化率，当使用低酶浓度时，生成木二糖和木三糖，而当使用高酶浓度时，则生成木糖和木二糖。
