Wu D J, Fujiwara H, Tanaka M, Onodera T, Matsuda M, Ishida M, Kawamura A, Takemura G, Fujiwara T, Nagano Y
Department of Internal Medicine and Pathology, Kyoto University, Japan.
Jpn Circ J. 1988 Apr;52(4):327-40. doi: 10.1253/jcj.52.327.
The distribution and progression of coronary arterial and aortic lesions were examined in 40 conventional Watanabe heritable hyperlipidemic (WHHL) rabbits. They were classified according to age into stage I (3-5 months old, 14 rabbits), stage II (6-12 months old; 12 rabbits) and stage III (14-28 months old; 14 rabbits). Fifteen normal Japanese rabbits served as controls. The findings obtained from serial and transverse sections of each of the extramural coronary arteries (ECA) and transverse sections of 4 to 5 equal pieces of whole ventricle for intramural coronary arteries (ICA) were quantified by an image analyzer. Atherosclerosis with positive Sudan III stain was seen in aorta, ECA and ICA over 200 mu in diameter. Atherosclerotic lesions were noted in the aortic arch in stage I rabbits and in the whole aorta in stages II and III rabbits. In ECA, stenosis due to atherosclerosis was noted in 14, 33 and 93% of stages I, II and III rabbits, respectively. Stenosis of over 75% in the orifices of the left and right coronary arteries was noted frequently (71%), while mural thrombi, hemorrhage, intimal rupture and recanalization were seen rarely. Striking features were non-atherosclerotic stenosis with negative Sudan III, seen in the ICA less than 200 mu in diameter of almost all the hearts of stages II and III rabbits. Acute and old myocardial infarction appeared in 5 of the 14 hearts of the stage III rabbits and the infarct-related ECA showed severe stenosis of over 90%. In conclusion, to detect coronary atherosclerosis, serial and transverse sections of ECA are needed. In conventional WHHL rabbits, the incidence of stenosis in ECA is very high, compared with that of the previous reports, and myocardial infarction is due to severe stenosis in ECA. Non-atherosclerotic lesions in ICA occur before the appearance of the atherosclerotic lesions in ECA.
在40只传统的渡边遗传性高脂血症（WHHL）兔中检查了冠状动脉和主动脉病变的分布及进展情况。根据年龄将它们分为I期（3 - 5个月龄，14只兔）、II期（6 - 12个月龄，12只兔）和III期（14 - 28个月龄，14只兔）。15只正常日本兔作为对照。通过图像分析仪对每只壁外冠状动脉（ECA）的连续和横切面以及壁内冠状动脉（ICA）的4至5个等份全心室横切面的检查结果进行定量分析。直径超过200μm的主动脉、ECA和ICA中可见苏丹III染色阳性的动脉粥样硬化。I期兔的主动脉弓出现动脉粥样硬化病变，II期和III期兔的整个主动脉出现病变。在ECA中，I期、II期和III期兔中分别有14%、33%和93%出现因动脉粥样硬化导致的狭窄。左右冠状动脉口狭窄超过75%的情况很常见（71%），而壁内血栓、出血、内膜破裂和再通则很少见。显著特征是在II期和III期兔几乎所有心脏直径小于200μm的ICA中出现苏丹III染色阴性的非动脉粥样硬化性狭窄。III期兔的14颗心脏中有5颗出现急性和陈旧性心肌梗死，与梗死相关的ECA显示严重狭窄超过90%。总之，为检测冠状动脉粥样硬化，需要ECA的连续和横切面。在传统的WHHL兔中，与先前报道相比，ECA狭窄的发生率非常高，心肌梗死是由ECA严重狭窄所致。ICA中的非动脉粥样硬化性病变在ECA动脉粥样硬化病变出现之前就已发生。
