College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China; State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, Donghua University, Shanghai 201620, China.
College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China.
Water Res. 2021 Jun 1;197:117095. doi: 10.1016/j.watres.2021.117095. Epub 2021 Mar 31.
A 300m/d demonstration project of soybean-process wastewater has been established recently with a Spiral Symmetric Stream Anaerobic Bioreactor (SSSAB) as the core. In order to obtain the optimal operation strategy for a full-scale SSSAB and to make it run efficiently and stably in a demonstration project, a Pilot-scale SSSAB (P-SSSAB, effective volume 100 L) was performed for the treatment of soybean-process wastewater over 216 days. The volumetric load rate (VLR) range of the P-SSSAB was 0.32~27.17 kg COD/(m·d), where the highest VLR [27.17 kg COD/(m·d)] was 2.01 times to the highest value [13.5 kg COD/(m·d)] reported. The pH and VFA/ALK of the effluent from the P-SSSAB were in the range of 6.9 up to 9.2 and 0.03 up to 0.17, respectively. The methane yield of the P-SSSAB increased from 0.03 m/kg COD to 0.47 m/kg COD, which was 3.36 times to the maximum value (0.14 m/kg COD) reported. To meet the influent requirement of the aerobic biological treatment in demonstration project (influent COD ≤ 1.5 g/L), the maximum VLR of SSSAB was optimal at about 22 kg COD/(m·d). By analyzing the sludge bed characteristics of the P-SSSAB, it was obvious that zone I (the bottom of the bed) was the major contributor of the COD removal, while zone III (the upper part of the bed) was the major contributor for the NH-N increase. The anaerobic granular sludge (AGS) in the bed showed a good granulation. The average MLVSS/MLSS value in sludge bed was about 0.7, and PN/PS in TB-EPS (zone I, II and III) increased to 6.830, 4.257, and 3.747, respectively. SMA and coenzyme F values of zone III were the maximum [666.35 ml CH/(g VSS·d) and 0.690 mol/g VSS, respectively]. According to the analysis obtained from the 16S rRNA high-throughput sequencing, the microbial community in the AGS had been more specific to the soybean-process wastewater since the bacteria Firmicutes were increased. The relative abundance of microbe which perform direct interspecies electron transfer (DIET) for the syntrophic degradation of VFAs and production of the methane has been increased significantly, such as the bacteria Syntrophomonas and archaea Methanosaeta.
最近建立了一个处理大豆加工废水的 300m³/d 示范工程，其核心是螺旋对称流厌氧生物反应器（SSSAB）。为了获得全尺寸 SSSAB 的最佳运行策略，并使其在示范工程中高效稳定运行，我们进行了一个中试规模的 SSSAB（P-SSSAB，有效体积 100L），以处理大豆加工废水，时间为 216 天。P-SSSAB 的容积负荷率（VLR）范围为 0.32-27.17kgCOD/（m·d），其中最高 VLR[27.17kgCOD/（m·d）]是报道的最高值[13.5kgCOD/（m·d）]的 2.01 倍。P-SSSAB 出水的 pH 值和 VFA/ALK 分别在 6.9 到 9.2 之间和 0.03 到 0.17 之间。P-SSSAB 的甲烷产量从 0.03m³/kgCOD 增加到 0.47m³/kgCOD，是报道的最大值（0.14m³/kgCOD）的 3.36 倍。为了满足示范工程好氧生物处理的进水要求（进水 COD≤1.5g/L），SSSAB 的最大 VLR 最佳约为 22kgCOD/（m·d）。通过分析 P-SSSAB 的污泥床特性，明显看出一区（床的底部）是 COD 去除的主要贡献者，而三区（床的上部）是 NH-N 增加的主要贡献者。床中的厌氧颗粒污泥（AGS）表现出良好的颗粒化。污泥床中 MLVSS/MLSS 的平均值约为 0.7，TB-EPS（一区、二区和三区）中的 PN/PS 分别增加到 6.830、4.257 和 3.747。三区的 SMA 和辅酶 F 值最大[666.35mlCH/（gVSS·d）和 0.690mol/gVSS]。根据 16S rRNA 高通量测序的分析，AGS 中的微生物群落对大豆加工废水的特异性更强，因为厚壁菌门的细菌数量增加。直接种间电子转移（DIET）用于 VFAs 的共代谢降解和甲烷产生的微生物的相对丰度显著增加，例如细菌 Syntrophomonas 和古菌 Methanosaeta。
