Bert V, Macnair M R, DE Laguerie P, Saumitou-Laprade P, Petit D
1 Laboratoire de Génétique et Evolution des Populations Végétales, UPRESA 8016, FR 1818, Bât SN2, Université de Lille 1, 59655 Villeneuve d'Ascq cedex, France.
New Phytol. 2000 May;146(2):225-233. doi: 10.1046/j.1469-8137.2000.00634.x.
Zinc tolerance was investigated in five populations of Arabidopsis halleri (syn.: Cardaminopsis halleri) raised from seeds collected from contaminated and uncontaminated sites. Tolerance was measured by determining the concentration which inhibited root growth (EC ). A. halleri populations from contaminated and uncontaminated sites were found to be Zn-tolerant compared with the Zn-nontolerant species Arabidopsis thaliana and A. lyrata subsp. petraea. At very high Zn concentrations, populations of A. halleri from uncontaminated sites were slightly less Zn-tolerant than those from contaminated sites. These observations support the hypothesis that in A. halleri, Zn tolerance is largely a constitutive property. One population from an uncontaminated site and one population from a contaminated site were studied for Zn uptake. Zinc content was measured in shoots and roots using a colorimetric test under laboratory conditions. The results showed that whatever their origin, individuals from both populations are Zn accumulators compared with the nonaccumulator species A. thaliana. Moreover, the population from the uncontaminated area accumulated Zn in its shoots and roots more quickly than the population from the contaminated site. These results suggest that, in A. halleri, Zn accumulation to very high concentration is a constitutive property.
对从受污染和未受污染地点采集的种子培育出的五个拟南芥种群（同义词：岩生庭荠）的锌耐受性进行了研究。通过测定抑制根系生长的浓度（EC）来衡量耐受性。与不耐锌的物种拟南芥和琴叶拟南芥相比，发现来自受污染和未受污染地点的岩生庭荠种群对锌具有耐受性。在锌浓度非常高时，来自未受污染地点的岩生庭荠种群的耐锌性略低于来自受污染地点的种群。这些观察结果支持了这样一种假设，即在岩生庭荠中，锌耐受性在很大程度上是一种固有特性。对来自未受污染地点的一个种群和来自受污染地点的一个种群进行了锌吸收研究。在实验室条件下，使用比色法测定地上部和根部的锌含量。结果表明，与非积累物种拟南芥相比，无论其来源如何，两个种群的个体都是锌积累者。此外，来自未受污染地区的种群在地上部和根部积累锌的速度比来自受污染地点的种群更快。这些结果表明，在岩生庭荠中，积累到非常高浓度的锌是一种固有特性。
