Vierheilig H, Bennett R, Kiddle G, Kaldorf M, Ludwig-Müller J
1 Institut für Phytopathologie, Christian-Albrechts-Universität, Hermann-Rodewald-Str. 9, D-24118 Kiel, Germany.
New Phytol. 2000 May;146(2):343-352. doi: 10.1046/j.1469-8137.2000.00642.x.
Under defined laboratory conditions it was shown that two glucosinolate-containing plant species, Tropaeolum majus and Carica papaya, were colonized by arbuscular mycorrhizal (AM) fungi, whereas it was not possible to detect AM fungal structures in other glucosinolate-containing plants (including several Brassicaceae). Benzylglucosinolate was present in all of the T. majus cultivars and in C. papaya it was the major glucosinolate. 2-Phenylethylglucosinolate was found in most of the non-host plants tested. Its absence in the AM host plants indicates a possible role for the isothiocyanate produced from its myrosinase-catalysed hydrolysis as a general AM inhibitory factor in non-host plants. The results suggest that some of the indole glucosinolates might also be involved in preventing AM formation in some of the species. In all plants tested, both AM hosts and non-hosts, the glucosinolate pattern was altered after inoculation with one of three different AM fungi (Glomus mosseae, Glomus intraradices and Gigaspora rosea), indicating signals between AM fungi and plants even before root colonization. The glucosinolate induction was not specifically dependent on the AM fungus. A time-course study in T. majus showed that glucosinolate induction was present during all stages of mycorrhizal colonization.
在特定的实验室条件下，研究表明，两种含硫代葡萄糖苷的植物——旱金莲和番木瓜，被丛枝菌根（AM）真菌定殖，而在其他含硫代葡萄糖苷的植物（包括几种十字花科植物）中未检测到AM真菌结构。所有旱金莲品种中均存在苄基硫代葡萄糖苷，在番木瓜中它是主要的硫代葡萄糖苷。在大多数测试的非寄主植物中发现了2-苯乙基硫代葡萄糖苷。在AM寄主植物中不存在该物质，这表明其由黑芥子酶催化水解产生的异硫氰酸酯可能作为非寄主植物中的一种普遍的AM抑制因子发挥作用。结果表明，一些吲哚硫代葡萄糖苷可能也参与了某些物种中AM形成的阻止过程。在所有测试的植物中，无论是AM寄主植物还是非寄主植物，接种三种不同的AM真菌（摩西球囊霉、根内球囊霉和玫瑰巨孢囊霉）之一后，硫代葡萄糖苷模式均发生了改变，这表明在根系定殖之前AM真菌与植物之间就存在信号传递。硫代葡萄糖苷的诱导并非特别依赖于AM真菌。在旱金莲中进行的一项时间进程研究表明，在菌根定殖的所有阶段均存在硫代葡萄糖苷的诱导现象。
