Johnson D, Leake J R, Read D J
Department of Animal and Plant Science, University of Sheffield, Sheffield S10 2TN, UK.
New Phytol. 2001 Dec;152(3):555-562. doi: 10.1046/j.0028-646X.2001.00273.x.
• A novel in-growth core system, enabling functional studies of natural communities of arbuscular mycorrhizal (AM) mycelia in soil is described and tested. • The cores have windows covered with nylon mesh of 35 µm pore size that prevent in-growth of roots but permit penetration of AM hyphae. They were inserted into grassland turf and contained either sterilized sand and a 'bait' seedling of Trifolium repens or nonsterile natural soil without bait plants. The impacts of hyphal severance, achieved by periodic rotation of some of the cores, upon AM colonization of bait plants (experiment 1) and transfer of P from soil to plants outside the cores (experiment 2) were examined. • Severance of AM hyphae reduced both AM colonization of bait plants and their shoot P concentrations. The shoot P concentrations of plants with mycelial access to PO -labelled cores were 10-fold greater than those which had no mycelial access. • It is concluded that this novel approach enables the functioning of mycorrhizal mycelial networks to be evaluated under conditions closely simulating those occurring in nature.
• 描述并测试了一种新型的内生核心系统，该系统能够对土壤中丛枝菌根（AM）菌丝的自然群落进行功能研究。
• 这些核心有覆盖着孔径为35微米尼龙网的窗口，可防止根系内生，但允许AM菌丝穿透。它们被插入草地草皮中，里面要么装有灭菌沙子和白三叶草“诱饵”幼苗，要么装有不含诱饵植物的非无菌天然土壤。通过定期旋转部分核心来实现菌丝切断，研究其对诱饵植物的AM定殖（实验1）以及磷从土壤向核心外植物转移（实验2）的影响。
• AM菌丝切断降低了诱饵植物的AM定殖及其地上部磷浓度。菌丝能够接触到³²P标记核心的植物，其地上部磷浓度比那些无法接触菌丝的植物高10倍。
• 得出结论，这种新方法能够在与自然条件极为相似的情况下评估菌根菌丝网络的功能。
