Ketelaar Tijs, Emons Anne Mie C
Laboratory of Plant Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands; Present address: Department of Biological Sciences, University of Durham, Science Laboratories, South Road, Durham DH1 3LE, UK.
New Phytol. 2001 Dec;152(3):409-418. doi: 10.1046/j.0028-646X.2001.00278.x.
In this review, we compare expansion of intercalary growing cells, in which growth takes place over a large surface, and root hairs, where expansion occurs at the tip only. Research that pinpoints the role of the cytoskeleton and the cytoplasmic free calcium in both root hairs and intercalary growing cells is reviewed. From the results of that research, we suggest experiments to be carried out on intercalary growing cells to test our hypotheses on plant cell expansion. Our main hypothesis is that instability of the cortical actin cytoskeleton determines the location where expansion takes place and the amount of expansion. Contents Summary 409 I. How do plant cells expand their surface? 409 II. Immunolocalization of epitopes in fixed root hairs for light-microscopy 410 III. The cytoskeleton in growing root hairs 412 1. Microtubules 412 2. Actin filaments 413 3. Free cytoplasmic calcium concentration 413 IV. The role of cytoskeletal elements and cytoplasmic free alcium in intercalary expanding root cells 414 1. Microtubules 414 2. Actin filaments 415 3. Free cytoplasmic calcium concentration 416 Acknowledgements 416 References 416.
在本综述中，我们比较了居间生长细胞（其生长发生在较大表面上）和根毛（其扩张仅发生在顶端）的扩展情况。本文综述了确定细胞骨架和细胞质游离钙在根毛和居间生长细胞中作用的研究。基于该研究结果，我们提出了针对居间生长细胞的实验，以检验我们关于植物细胞扩展的假设。我们的主要假设是，皮层肌动蛋白细胞骨架的不稳定性决定了扩展发生的位置和扩展量。内容摘要409 一、植物细胞如何扩展其表面？409 二、用于光学显微镜观察的固定根毛中表位的免疫定位410 三、生长中的根毛中的细胞骨架412 1. 微管412 2. 肌动蛋白丝413 3. 游离细胞质钙浓度413 四、细胞骨架成分和细胞质游离钙在居间扩展根细胞中的作用414 1. 微管414 2. 肌动蛋白丝415 3. 游离细胞质钙浓度416 致谢416 参考文献416
