Molina Randy, Smith Jane E, McKay Donaraye, Melville L H
U.S. Department of Agriculture, Forest Service, Pacific Northwest Research Station, Forestry Sciences Laboratory, 3200 Jefferson Way, Corvallis, OR 97331, USA.
Department of Botany, University of Guelph. Guelph, Ontario, NIG 2W1, Canada.
New Phytol. 1997 Nov;137(3):519-528. doi: 10.1046/j.1469-8137.1997.00836.x.
Seedlings of Pseudotsuga menziesii (Mirb.) Franco, Pinus ponderosa Dougl. ex Laws, Arbutus menziesii Pursh., and cuttings of Arclast apliylos uva-ursi (L.) Spreng were grown in monoculture and in conifer-hardwood dual-culture combinations in the glasshouse and inoculated with spore slurries of six Rhizopogon species. The primary objectives were to assess and compare the pattern of host specificity between symbionts and to study the influence of co-cultured plants on ecromycorrhiza development. The Rhizopogon spp. ranged from genus-specific to multiple-host compatible. In monoculture, four Rhisopogon sp, (R. ellenae Smith, R. occidentalis Zeller & Dodge, R. smithii Hosfnrd and R. subcaerulescens Smith) formed ectomycorrhizas with Pinus ponderosa, and two Rhizopogon sp. (R. parksii Smith and R. vinicolor Smith) formed ectomycorrhizas with Pseudotsuga menziesii. None of the fungi tested developed ectomycorrhizas on Arbutus menziesii or Arctostaphylos uvaursi in monoculture. In dual culture, three of the four Rhizopogon species (R. ellenae, R. occidentalis and R. subcaerulescens) that formed ectomycorrhizas on Pinus ponderosa, formed some ectomycorrhizas on Arbutus menziesii and Arrtastaphvlos uva-ursi. Rhizopogon parksii and R. tinicoior only formed ectomycorrhizas on Pseudotsuga mensiesti.
对花旗松（Pseudotsuga menziesii (Mirb.) Franco）、西黄松（Pinus ponderosa Dougl. ex Laws）、草莓树（Arbutus menziesii Pursh.）的幼苗以及熊果（Arclast apliylos uva - ursi (L.) Spreng）的插条进行了温室单培养和针叶树 - 硬木双培养组合种植，并接种了六种须腹菌属真菌的孢子悬浮液。主要目的是评估和比较共生体之间宿主特异性的模式，并研究共培养植物对外生菌根发育的影响。须腹菌属真菌的宿主范围从属特异性到多宿主兼容。在单培养中，四种须腹菌（埃氏须腹菌（Rhizopogon ellenae Smith）、西方须腹菌（R. occidentalis Zeller & Dodge）、史氏须腹菌（R. smithii Hosfnrd）和近蓝须腹菌（R. subcaerulescens Smith））与西黄松形成了外生菌根，两种须腹菌（帕克须腹菌（R. parksii Smith）和酒红须腹菌（R. vinicolor Smith））与花旗松形成了外生菌根。在单培养中，所测试的真菌均未在草莓树或熊果上形成外生菌根。在双培养中，在西黄松上形成外生菌根的四种须腹菌中的三种（埃氏须腹菌、西方须腹菌和近蓝须腹菌）在草莓树和熊果上形成了一些外生菌根。帕克须腹菌和酒红须腹菌仅在花旗松上形成外生菌根。
