Utriainen M A, Kärenlampi L V, Kärenlampi S O, Schat H
Department of Biochemistry and Biotechnology, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland.
Department of Ecology and Environmental Science, University of Kuopio, P.O. Box 1627 FIN-70211 Kuopio, Finland.
New Phytol. 1997 Nov;137(3):543-549. doi: 10.1046/j.1469-8137.1997.00840.x.
Copper and zinc tolerances of 10 micropropagated birch (Betula pendula and B. pubescens) clones were studied in hydroponic culture. Tolerance indices were determined, based on the mean growth rate of the longest root in 1 wk. A seed-derived clone (142A), from a lead/Zn-contaminated site showed more tolerance to Cu and Zn than bud-derived clones (HA02 and HA18) from a Cu/nickel-contaminated site or an ozone-tolerant clone (KL-2-M) from an uncontaminated area. For Cu, the EC values were 30, 14, 8 and 11 μM in clones 142A, HA02, HA18 and KL-2-M, respectively. FOT Zn, the EC s were 4000 and 350 μM in clones 142A and KL-2-M, respectively. The relative Cu and Zn tolerances of the other clones were estimated by growing the plants in 30 μ CuSO , and in 2000 or 350 μM ZnSO , respectively. It is of interest that the Zn-tolerant clone 142A was tolerant to Cu, although this metal was present at a very low concentration in the soil where the parent tree grows. Another clone (142B), from another seed of the same parent tree, was tolerant neither to Zn nor Cu. Compared with their own EC s for root growth for Cu, 142A took up more Cu than KL-2-M, suggesting that the higher tolerance of the former clone is not explained by reduced uptake of Cu. The Zn uptake in clones 142A and KL-2-M was studied at 4000 μM and 800 μM Zn, respectively. Interestingly, the roots of both clones contained the same amount of Zn, even though clone 142A was exposed to a fivefold concentration of Zn.
在水培条件下研究了10个微繁殖桦树（垂枝桦和毛桦）无性系对铜和锌的耐受性。根据最长根在1周内的平均生长速率确定耐受性指标。来自铅/锌污染场地的种子衍生无性系（142A）对铜和锌的耐受性高于来自铜/镍污染场地的芽衍生无性系（HA02和HA18）或来自未污染地区的耐臭氧无性系（KL-2-M）。对于铜，无性系142A、HA02、HA18和KL-2-M的EC值分别为30、14、8和11μM。对于锌，无性系142A和KL-2-M的EC值分别为4000和350μM。通过分别在30μM硫酸铜和2000或350μM硫酸锌中种植植物，估计了其他无性系对铜和锌的相对耐受性。有趣的是，耐锌无性系142A对铜也具有耐受性，尽管这种金属在母树生长的土壤中含量非常低。来自同一母树另一颗种子的另一个无性系（142B）对锌和铜均无耐受性。与它们自身根生长对铜的EC值相比，142A比KL-2-M吸收了更多的铜，这表明前一个无性系较高的耐受性并非由铜吸收减少所致。分别在4000μM和800μM锌浓度下研究了无性系142A和KL-2-M对锌的吸收。有趣的是，尽管无性系142A暴露于五倍浓度的锌中，但两个无性系的根中锌含量相同。
