National Institute of Environmental Health Sciences, Comparative & Molecular Pathogenesis Branch, National Toxicology Program, Durham, North Carolina;, Email:
Charles River Laboratories, Durham, North Carolina.
Comp Med. 2021 Jun 1;71(3):191-202. doi: 10.30802/AALAS-CM-20-000104. Epub 2021 Apr 16.
Systemic buprenorphine and topical antiseptics such as chlorhexidine are frequently used in research animals to aid in pain control and to reduce infection, respectively. These therapeutics are controversial, especially when used in wound healing studies, due to conflicting data suggesting that they delay wound healing. Low-level laser therapy (LLLT) has been used to aid in wound healing without exerting the systemic effects of therapies such as buprenorphine. We conducted 2 studies to investigate the effects of these common treatment modalities on the rate of wound healing in mice. The first study used models of punch biopsy and dermal abrasion to assess whether buprenorphine HCl or 0.12% chlorhexidine delayed wound healing. The second study investigated the effects of sustained-released buprenorphine, 0.05% chlorhexidine, and LLLT on excisional wound healing. The rate of wound healing was assessed by obtaining photographs on days 0, 2, 4, 7, and 9 for the punch biopsy model in study 1, days 0, 1, 2, 4, 6, 8, 11, and 13 for the dermal abrasion model in study 1, and days 0, 3, 6, and 10 for the mice in study 2. Image J software was used to analyze the photographed wounds to determine the wound area. When comparing the wound area on the above days to the original wound area, no significant differences in healing were observed for any of the treatment groups at any time period for either study. Given the results of these studies, we believe that systemic buprenorphine, topical chlorhexidine, and LLLT can be used without impairing or delaying wound healing in mice.
全身给予丁丙诺啡和局部使用抗菌剂如洗必泰通常用于研究动物以帮助控制疼痛和减少感染，分别。这些治疗方法存在争议，尤其是在伤口愈合研究中，因为有相互矛盾的数据表明它们会延迟伤口愈合。低水平激光疗法（LLLT）已被用于促进伤口愈合而不会产生丁丙诺啡等治疗方法的全身作用。我们进行了两项研究，以调查这些常见治疗方式对小鼠伤口愈合速度的影响。第一项研究使用打孔活检和真皮擦伤模型评估盐酸丁丙诺啡或 0.12%洗必泰是否延迟了伤口愈合。第二项研究调查了持续释放丁丙诺啡、0.05%洗必泰以及 LLLT 对切除伤口愈合的影响。通过在研究 1 中对打孔活检模型在第 0、2、4、7 和 9 天以及在研究 1 中对真皮擦伤模型在第 0、1、2、4、6、8、11 和 13 天获得照片来评估伤口愈合的速度，并在研究 2 中对小鼠在第 0、3、6 和 10 天获得照片。使用 Image J 软件分析拍摄的伤口以确定伤口面积。当将上述各天的伤口面积与原始伤口面积进行比较时，在任何研究的任何时间段，任何治疗组的愈合均无明显差异。鉴于这些研究的结果，我们认为全身给予丁丙诺啡、局部使用洗必泰和 LLLT 可在不损害或延迟小鼠伤口愈合的情况下使用。
