Gammel Jessie E, Biskup Jeffery J, Drum Marti G, Newkirk Kim, Lux Cassie N
The University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee.
Vet Surg. 2018 May;47(4):499-506. doi: 10.1111/vsu.12795. Epub 2018 Apr 14.
To determine the effects of low-level laser therapy (LLLT) on primarily closed incisions and full thickness open wounds in dogs.
Prospective, masked, placebo-controlled design.
Healthy intact female dogs (n = 10).
Dogs underwent bilateral flank ovariectomy procedures, and open wounds were created bilaterally with a punch biopsy. Each side of the dog (incision and open wound) was randomly assigned to the treatment (TX) group or the control (CN) group. The TX group received LLLT once daily for 5 days with a 980-nm laser and a total energy density of 5 J/cm . The CN group received a sham treatment (laser turned off) for an identical amount of time each day. The wounds were assessed visually; measured; photographed at postoperative days 3, 7, 11, and 14; and biopsied on postoperative days 7 and 14. A 2-way repeated measures multivariate analysis of variance was used to analyze differences between groups.
There was no difference between groups for subjective assessment of healing time and wound measurements (P = .7). There was no difference in histopathologic assessment except that the CN group had more necrosis and perivascular lymphocytes and macrophages at day 7 (P = .03). The TX group had more perivascular lymphocytes and macrophages at day 14 (P = .01).
LLLT did not appear to influence the healing of surgically created incisions and small wounds with the methodology reported here.
Results of this study do not support recommending LLLT to stimulate healing of uncomplicated, small wounds and incisions.
确定低强度激光疗法（LLLT）对犬类一期闭合切口和全层开放性伤口的影响。
前瞻性、盲法、安慰剂对照设计。
健康未绝育雌性犬（n = 10）。
犬接受双侧胁腹卵巢切除术，并通过打孔活检在双侧制造开放性伤口。犬的每一侧（切口和开放性伤口）随机分为治疗（TX）组或对照（CN）组。TX组使用980纳米激光，每天接受一次LLLT治疗，持续5天，总能量密度为5焦/平方厘米。CN组每天在相同时间接受假治疗（激光关闭）。对伤口进行视觉评估；测量；在术后第3、7、11和14天拍照；并在术后第7天和14天进行活检。采用双向重复测量多变量方差分析来分析组间差异。
在愈合时间的主观评估和伤口测量方面，两组之间没有差异（P = 0.7）。组织病理学评估没有差异，只是CN组在第7天有更多坏死以及血管周围淋巴细胞和巨噬细胞（P = 0.03）。TX组在第14天有更多血管周围淋巴细胞和巨噬细胞（P = 0.01）。
采用本文报道的方法，LLLT似乎并未影响手术造成的切口和小伤口的愈合。
本研究结果不支持推荐使用LLLT来促进简单小伤口和切口的愈合。
