Ekici Ömer, Aslan Esra, Aladağ Tuğçe, Güzel Hilal, Korkmaz Ömer Adil, Bostancı Aykut, Sadi Gökhan, Pektaş Mehmet Bilgehan
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey.
J Food Biochem. 2022 Mar;46(3):e13732. doi: 10.1111/jfbc.13732. Epub 2021 Apr 16.
The aim of the study was to evaluate whether high-fructose corn syrup (HFCS) intake (20% beverages) impacts antioxidative structures and inflammation in the gingival tissue and masseter muscle of rats. Kefir was tested for its potential utility on changes induced by HFCS. Animals were randomly divided into four groups as control, kefir, HFCS, and HFCS plus kefir. HFCS was given as 20% solutions in drinking water while kefir supplementations were given by gastric gavage for 8 weeks. It has been clearly determined that the HFCS diet increased expressions of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α proinflammatory structures via lymphocyte infiltration by suppressing antioxidant enzymes such as catalase, superoxide dismutase, and glutathione peroxidase in both tissues. Kefir improved these undesirable changes in rats fed with HFCS. The results of this current study, the first investigation to examine the effects of kefir on masseter muscle and gingival tissue, may provide new access to the restorative effects of kefir consumption on oral health disorders caused by high fructose in the diet. PRACTICAL APPLICATIONS: In this study, at an early age, the effects of kefir on improving inflammation via antioxidation in the masseter muscle and gingival tissue were investigated for the first time. We showed that kefir feeding ameliorates lymphocyte infiltration on the high-fructose corn syrup (HFCS)-induced masseter muscle and gingival tissue inflammation in rats. The mRNA expressions of inflammatory parameters measured in the study were supported by protein measurements via ELISA or immunohistochemistry. In the present study, kefir may play an important role in the antioxidation and inflammation process on the masseter muscle and gingival tissue against HFCS.
本研究的目的是评估高果糖玉米糖浆（HFCS）摄入量（20%饮料）是否会影响大鼠牙龈组织和咬肌的抗氧化结构及炎症反应。对开菲尔改善HFCS诱导变化的潜在效用进行了测试。动物被随机分为四组：对照组、开菲尔组、HFCS组和HFCS加开菲尔组。HFCS以20%的溶液形式添加到饮用水中，而开菲尔则通过灌胃补充8周。已明确确定，HFCS饮食通过淋巴细胞浸润增加白细胞介素（IL）-6、IL-1β和肿瘤坏死因子-α等促炎结构的表达，同时抑制两种组织中的过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶等抗氧化酶。开菲尔改善了喂食HFCS大鼠的这些不良变化。本研究首次调查了开菲尔对咬肌和牙龈组织的影响，其结果可能为食用开菲尔对饮食中高果糖引起的口腔健康问题的恢复作用提供新的途径。实际应用：在本研究中，首次在幼年时研究了开菲尔通过抗氧化作用改善咬肌和牙龈组织炎症的效果。我们发现，喂食开菲尔可减轻高果糖玉米糖浆（HFCS）诱导的大鼠咬肌和牙龈组织炎症中的淋巴细胞浸润。通过ELISA或免疫组织化学进行的蛋白质测量支持了研究中测量的炎症参数的mRNA表达。在本研究中，开菲尔可能在针对HFCS的咬肌和牙龈组织抗氧化及炎症过程中发挥重要作用。
