Khurana Preeti, Kolundzic Nikola, Flohr Carsten, Ilic Dusko
Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK.
Assisted Conception Unit, Guy's Hospital, London, UK.
Exp Dermatol. 2021 Nov;30(11):1572-1587. doi: 10.1111/exd.14358. Epub 2021 Jun 4.
To effectively study the skin and its pathology, various platforms have been used to date, with in vitro 3D skin models being considered the future gold standard. These models have generally been engineered from primary cell lines. However, their short life span leading to the use of various donors, imposes issues with genetic variation. Human pluripotent stem cell (hPSC)-technology holds great prospects as an alternative to the use of primary cell lines to study the pathophysiology of human skin diseases. This is due to their potential to generate an unlimited number of genetically identical skin models that closely mimic the complexity of in vivo human skin. During the past decade, researchers have therefore started to use human embryonic and induced pluripotent stem cells (hESC/iPSC) to derive skin resident-like cells and components. These have subsequently been used to engineer hPSC-derived 3D skin models. In this review, we focus on the advantages, recent developments, and future perspectives in using hPSCs as an alternative cell source for modelling human skin diseases in vitro.
为了有效地研究皮肤及其病理学，迄今为止已使用了各种平台，其中体外3D皮肤模型被认为是未来的黄金标准。这些模型通常由原代细胞系构建而成。然而，它们的寿命较短，导致需要使用不同的供体，这带来了基因变异的问题。人类多能干细胞（hPSC）技术作为替代原代细胞系来研究人类皮肤疾病病理生理学的方法，具有广阔的前景。这是因为它们有潜力生成无限数量的基因相同的皮肤模型，这些模型能紧密模拟体内人类皮肤的复杂性。因此，在过去十年中，研究人员开始使用人类胚胎干细胞和诱导多能干细胞（hESC/iPSC）来衍生皮肤驻留样细胞和成分。这些随后被用于构建hPSC衍生的3D皮肤模型。在本综述中，我们重点关注使用hPSC作为体外模拟人类皮肤疾病的替代细胞来源的优势、最新进展和未来前景。
