Department of Histology and Embryology, Erciyes University, Faculty of Medicine, 38039, Kayseri, Turkey.
Department of Pharmacology, Erciyes University, Faculty of Medicine, 38039, Kayseri, Turkey.
J Chem Neuroanat. 2021 Jul;114:101958. doi: 10.1016/j.jchemneu.2021.101958. Epub 2021 Apr 14.
We aimed to investigate the effects of umbilical cord-derived mesenchymal stem cells and erythropoietin on nerve regeneration in the sciatic nerve 'crush injury' in a rat model.
Experimental animals were randomly divided into 5 groups: Crush Injury, Sham, Crush Injury + Erythropoietin, Crush Injury + Mesenchymal Stem Cell, Crush Injury + Erythropoietin + Mesenchymal Stem Cell groups. Crush injury made with bulldog clamp. Mesencyhmal stem cells delivered by enjection locally. Erythropoietin administered by intraperitoneally. On the 0th, 14th and 28th days, all groups underwent a sciatic functional index test. On 28th day, sciatic nerves were harvested and histopathological appearance, axon number and axon diameter of the sciatic nerves were evaluated with Oil Red O staining. Immunoreactivity of nerve growth factor, neurofilament-H and caspase-3 were determined by immunofluorescence staining in nerve tissue.
In histopathological examination, axons and nerve bundles exhibiting normal nerve architecture in the Sham group. Crush Injury + Mesenchymal Stem Cell group has similar histological appearance to the Sham group. The number of axons were higher in the Mesenchymal Stem Cell groups compared to the Crush Injury group. Nerve growth factor immunoreactivity intensity was significantly lower in Crush Injury + Mesenchymal Stem Cell group compared to Crush Injury group. Neurofilament-H density was higher in the treatment groups when compared to the Crush Injury group.
In this study, it was found that umbilical cord-derived mesenchymal stem cells and erythropoietin treatments effects positively regeneration of crush injury caused by bulldog clamp in the sciatic nerve of rats.
我们旨在研究脐带间充质干细胞和促红细胞生成素对大鼠坐骨神经挤压伤模型中神经再生的影响。
实验动物随机分为 5 组：挤压伤组、假手术组、挤压伤+促红细胞生成素组、挤压伤+间充质干细胞组、挤压伤+促红细胞生成素+间充质干细胞组。使用虎钳造成挤压伤。局部注射间充质干细胞。促红细胞生成素腹腔内给药。在第 0、14 和 28 天，所有组均进行坐骨神经功能指数测试。在第 28 天，收获坐骨神经，用油红 O 染色评估坐骨神经的组织病理学表现、轴突数量和轴突直径。通过免疫荧光染色在神经组织中测定神经生长因子、神经丝-H 和半胱天冬酶-3 的免疫反应性。
在组织病理学检查中，假手术组的轴突和神经束表现出正常的神经结构。挤压伤+间充质干细胞组具有与假手术组相似的组织学表现。与挤压伤组相比，间充质干细胞组的轴突数量更高。与挤压伤组相比，挤压伤+间充质干细胞组的神经生长因子免疫反应性强度显著降低。与挤压伤组相比，治疗组的神经丝-H 密度更高。
在这项研究中，发现脐带间充质干细胞和促红细胞生成素治疗可促进大鼠坐骨神经挤压伤模型中由虎钳引起的挤压伤后的再生。
