Posgrado en Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Av. Ciudad Universitaria 3000, C.P. 04510, Coyoacán, Ciudad de México, Mexico; Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Prolongación Avenida Niños Héroes S/N, Puerto Morelos, Quintana Roo, 77580, Mexico.
Unidad Multidisciplinaria de Docencia e Investigación de Sisal, Facultad de Ciencias, Universidad Nacional Autónoma de México, Yucatán, Mexico.
Anim Reprod Sci. 2021 May;228:106749. doi: 10.1016/j.anireprosci.2021.106749. Epub 2021 Apr 1.
The aim of this study was to evaluate egg production and quality of females of the Pacific white shrimp, Litopenaeus vannamei, in which there was or was not unilateral eyestalk ablation after there was pre-maturation culturing in biofloc or clear-water systems. Acylglycerides, cholesterol, glucose and total soluble protein were determined for the hepatopancreas, ovaries, hemolymph and eggs. Females cultured using the biofloc system had a larger number of eggs released per spawning and per gram of spawning specimen body weight. The number of total spawning's per week was comparable among treatments. Females cultured in the biofloc system in which there was no eyestalk ablation had that greatest concentrations of nutrient reserves in the hepatopancreas (P < 0.05) with the females cultured in the biofloc and clear-water system that had eye stalk ablation having the next most abundant nutrient reserves. There were the least concentrations of nutrient reserves in females with eyestalk ablation cultured in the clear-water system (P < 0.05). There, however, were no difference in nutrient reserve concentrations in the hemolymph and ovaries. In the eggs, there was the same trend among treatments as the hepatopancreas nutrient reserves, indicating that both eyestalk ablation and pre-maturation culture conditions (i.e., either biofloc or clear-water) affected the quality of eggs in L. vannamei.
本研究旨在评估太平洋白对虾（Litopenaeus vannamei）雌虾在生物絮团或清水系统中进行预成熟培养后单侧切除眼柄对其产卵和卵子质量的影响。对肝胰腺、卵巢、血淋巴和卵子中的酰基甘油、胆固醇、葡萄糖和总可溶性蛋白进行了测定。在生物絮团系统中培养的雌虾每次产卵和每克产卵标本体重释放的卵子数量更多。每周总产卵次数在各处理组之间相当。未切除眼柄的生物絮团系统中培养的雌虾肝胰腺中营养储备的浓度最高（P < 0.05），而在生物絮团和切除眼柄的清水系统中培养的雌虾具有下一个最丰富的营养储备。在切除眼柄的清水系统中培养的雌虾中营养储备的浓度最低（P < 0.05）。然而，在血淋巴和卵巢中，营养储备浓度没有差异。在卵子中，与肝胰腺营养储备的趋势相同，表明眼柄切除和预成熟培养条件（即生物絮团或清水）都影响了 L. vannamei 的卵子质量。
