School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.
Laboratory of Mass Spectrometry, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.
J Assist Reprod Genet. 2021 Aug;38(8):2209-2217. doi: 10.1007/s10815-021-02189-y. Epub 2021 Apr 18.
The aim of this study was to analyze the metabolic profiles of blastocoel fluid (BF) obtained from bovine embryos produced in vivo and in vitro.
Expanded blastocysts (20/group) that were in vitro and in vivo derived at day 7 were used. BF was collected and analyzed under direct infusion conditions using a microTOF-Q mass spectrometer with electrospray ionization and a mass range of 50-650 m/z.
The spectrometry showed an evident difference in the metabolic profiles of BF from in vivo and in vitro produced embryos. These differences were very consistent between the samples of each group suggesting that embryo fluids can be used to identify the origin of the embryo. Ions 453.15 m/z, 437.18 m/z, and 398.06 m/z were identified as biomarkers for the embryo's origin with 100% sensitivity and specificity. Although it was not possible to unveil the molecular identity of the differential ions, the resulting spectrometric profiles provide a phenotype capable of differentiating embryos and hence constitute a potential parameter for embryo selection.
To the best of our knowledge, our results showed, for the first time, an evident difference between the spectrometric profiles of the BF from bovine embryos produced in vivo and in vitro.
本研究旨在分析体内和体外培养的牛胚胎囊胚腔液（BF）的代谢谱。
使用第 7 天体内和体外扩增的囊胚（每组 20 个）。在直接注入条件下，使用带有电喷雾电离和 50-650 m/z 质量范围的 microTOF-Q 质谱仪收集和分析 BF。
质谱显示体内和体外培养的胚胎 BF 的代谢谱存在明显差异。这些差异在每组的样本之间非常一致，表明胚胎液可用于识别胚胎的来源。离子 453.15 m/z、437.18 m/z 和 398.06 m/z 被鉴定为胚胎来源的生物标志物，具有 100%的灵敏度和特异性。尽管无法揭示差异离子的分子身份，但所得的光谱谱图提供了一种能够区分胚胎的表型，因此构成了胚胎选择的潜在参数。
据我们所知，我们的研究结果首次显示了体内和体外培养的牛胚胎 BF 的光谱谱图之间存在明显差异。
