使用改良的亚胺培南/瑞来巴坦Carba NP试验快速检测产KPC的肠杆菌科细菌。
Rapid detection of KPC-producing Enterobacterales by using a modified Carba NP test with imipenem/relebactam.
作者信息
Oviaño Marina, Ramírez Cecilia de la Luna, González-Bardanca Mónica, Candela Ana, Bou Germán
机构信息
Servicio de Microbiología. Complejo Hospitalario Universitario A Coruña, La Coruña. Spain.
Servicio de Microbiología. Hospital General Universitario Gregorio Marañón, Madrid, Spain.
出版信息
Enferm Infecc Microbiol Clin (Engl Ed). 2021 Apr 15. doi: 10.1016/j.eimc.2021.03.013.
INTRODUCTION
Here, we propose a novel modified Carba NP test for detecting KPC-producing Enterobacterales using imipenem/relebactam.
MATERIAL AND METHODS
The test performance was evaluated in a random selection of 160 previously molecularly characterized clinical isolates carrying the 110 bla, 1 bla, 12 bla, 4 bla, 3 bla and 42 bla genes. The proposed method relies on the detection of imipenem hydrolysis in an imipenem/relebactam antibiotic solution and subsequent visual interpretation by color change.
RESULTS
All class A producing Enterobacterales (111/111) were detected using imipenem/relebactam as no visual appreciation of color change was perceived due to a nule hydrolysis of imipenem in the antibiotic solution. Overall, the assay showed 100% sensitivity (111/111) and specificity (69/69) for detecting class A KPC-producing Enterobacterales.
DISCUSSION
The biochemical assay provides very reliable results for detecting KPC-producing Enterobacterales, with a turnaround time of less than 1 hour, minimum handling and no specialized equipment required.
引言
在此，我们提出一种新型改良Carba NP试验，用于使用亚胺培南/雷利巴坦检测产KPC的肠杆菌科细菌。
材料与方法
在随机选择的160株先前经分子特征鉴定的临床分离株中评估该试验性能，这些分离株携带blaKPC-1、blaKPC-2、blaKPC-3、blaKPC-4、blaKPC-5和blaKPC-12基因。所提出的方法依赖于检测亚胺培南/雷利巴坦抗生素溶液中亚胺培南的水解情况，随后通过颜色变化进行目视判读。
结果
使用亚胺培南/雷利巴坦检测到所有产A类酶的肠杆菌科细菌（111/111），因为抗生素溶液中亚胺培南的水解未观察到颜色变化。总体而言，该检测方法在检测产A类KPC的肠杆菌科细菌时显示出100%的敏感性（111/111）和特异性（69/69）。
讨论
该生化检测方法在检测产KPC的肠杆菌科细菌时提供了非常可靠的结果，周转时间不到1小时，操作简便，无需专门设备。
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