含有未配对碱基的15聚体DNA双链体的晶体结构
Crystal structure of 15-mer DNA duplex containing unpaired bases.
作者信息
Miller M, Harrison R W, Wlodawer A, Appella E, Sussman J L
机构信息
Crystallography Laboratory, NCI-Frederick Cancer Research Facility, Maryland 21701.
出版信息
Nature. 1988 Jul 7;334(6177):85-6. doi: 10.1038/334085a0.
Errors during DNA replication or repair can lead to the presence of unpaired or inserted bases in the double helix, as well as to mismatched base pairs. So far only structures of the latter type have been characterized by X-ray crystallography. We report here a 3-A crystal structure of DNA 15-mer d(CGCGAAATTTACGCG), which forms a duplex with two unpaired adenine residues looped outside the B-type helix. This arrangement is in disagreement with the nuclear magnetic resonance spectroscopy results for the same 15-mer in solution, indicating polymorphic nature of the structure adopted by this sequence.
DNA复制或修复过程中的错误会导致双螺旋中出现未配对或插入的碱基，以及错配的碱基对。到目前为止，只有后一种类型的结构通过X射线晶体学进行了表征。我们在此报告DNA 15聚体d（CGCGAAATTTACGCG）的3埃晶体结构，它形成了一个双链体，两个未配对的腺嘌呤残基在B型螺旋外部成环。这种排列与该15聚体在溶液中的核磁共振光谱结果不一致，表明该序列所采用结构的多态性。
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