Ryvkin Julia, Bentzur Assa, Shmueli Anat, Tannenbaum Miriam, Shallom Omri, Dokarker Shiran, Benichou Jennifer I C, Levi Mali, Shohat-Ophir Galit
The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
The Leslie and Susan Gonda Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan, Israel.
Front Behav Neurosci. 2021 Mar 31;15:628662. doi: 10.3389/fnbeh.2021.628662. eCollection 2021.
Social behaviors are mediated by the activity of highly complex neuronal networks, the function of which is shaped by their transcriptomic and proteomic content. Contemporary advances in neurogenetics, genomics, and tools for automated behavior analysis make it possible to functionally connect the transcriptome profile of candidate neurons to their role in regulating behavior. In this study we used to explore the molecular signature of neurons expressing receptor for neuropeptide F (NPF), the fly homolog of neuropeptide Y (NPY). By comparing the transcription profile of NPFR neurons to those of nine other populations of neurons, we discovered that NPFR neurons exhibit a unique transcriptome, enriched with receptors for various neuropeptides and neuromodulators, as well as with genes known to regulate behavioral processes, such as learning and memory. By manipulating RNA editing and protein ubiquitination programs specifically in NPFR neurons, we demonstrate that the proper expression of their unique transcriptome and proteome is required to suppress male courtship and certain features of social group interaction. Our results highlight the importance of transcriptome and proteome diversity in the regulation of complex behaviors and pave the path for future dissection of the spatiotemporal regulation of genes within highly complex tissues, such as the brain.
社会行为由高度复杂的神经元网络的活动介导，其功能由它们的转录组和蛋白质组内容塑造。神经遗传学、基因组学以及自动行为分析工具的当代进展使得将候选神经元的转录组谱与其在调节行为中的作用进行功能连接成为可能。在本研究中，我们使用[具体方法未给出]来探索表达神经肽F（NPF）受体的神经元的分子特征，神经肽F是神经肽Y（NPY）的果蝇同源物。通过将NPFR神经元的转录谱与其他九种神经元群体的转录谱进行比较，我们发现NPFR神经元呈现出独特的转录组，富含各种神经肽和神经调节剂的受体，以及已知调节行为过程（如学习和记忆）的基因。通过特异性地在NPFR神经元中操纵RNA编辑和蛋白质泛素化程序，我们证明其独特转录组和蛋白质组的正确表达对于抑制雄性求偶和社会群体互动的某些特征是必需的。我们的结果突出了转录组和蛋白质组多样性在复杂行为调节中的重要性，并为未来剖析高度复杂组织（如大脑）内基因的时空调节铺平了道路。
