低浓度的苯环己哌啶选择性地阻断培养海马神经元中持续的而非瞬时的电压依赖性钾电流。

Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons.

作者信息

ffrench-Mullen J M, Rogawski M A, Barker J L

机构信息

Laboratory of Neurophysiology, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.

出版信息

Neurosci Lett. 1988 Jun 7;88(3):325-30. doi: 10.1016/0304-3940(88)90232-7.

DOI:10.1016/0304-3940(88)90232-7

PMID:3386879

Abstract

We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (less than 50-100 microM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 microM, respectively.

摘要

我们研究了苯环己哌啶（PCP）对培养的胚胎大鼠海马神经元电压依赖性钾离子电流的影响。在存在河豚毒素以阻断钠离子电流的情况下进行全细胞电压钳记录。去极化电压阶跃激活了两个外向电流成分：（i）一个快速激活和失活（“瞬时”）成分，IA，以及（ii）一个缓慢激活、最小失活（“持续”）成分，IK。在低浓度（小于50 - 100微摩尔）时，PCP对IK产生选择性、可逆性阻断，对IA影响最小；然而，在较高浓度时，两种电流均被抑制。IK和IA阻断的半数抑制浓度（IC50）分别为36和310微摩尔。