Ouyang Ying, Chen Shuyu, Zhao Liqing, Song Yiting, Lei Anping, He Jiayi, Wang Jiangxin
Shenzhen Key Laboratory of Marine Bioresources and Eco-Environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.
College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, China.
Front Bioeng Biotechnol. 2021 Mar 31;9:652021. doi: 10.3389/fbioe.2021.652021. eCollection 2021.
The microalga is utilized in the food, medicinal, and supplement industries. However, its mass production is currently limited by its low production efficiency and high risk of microbial contamination. In this study, physiological and biochemical parameters of co-cultivated with the bacteria were investigated. A previous study reports the benefits of and co-cultivation; however, no bacterium growth and molecular mechanisms were further investigated. Our results show that this co-cultivation positively increased total chlorophyll, microalgal growth, dry weight, and storage sugar paramylon content of compared to the pure culture without . This analysis represents the first comprehensive metabolomic study of microalgae-bacterial co-cultivation, with 339 metabolites identified. This co-cultivation system was shown to have synergistic metabolic interactions between microalgal and bacterial cells, with a significant increase in methyl carbamate, ectoine, choline, methyl N-methylanthranilate, gentiatibetine, 4R-aminopentanoic acid, and glu-val compared to the cultivation of alone. Taken together, these results fill significant gaps in the current understanding of microalgae-bacteria co-cultivation systems and provide novel insights into potential improvements for mass production and industrial applications of .
微藻被应用于食品、医药和补充剂行业。然而，其大规模生产目前受到低生产效率和高微生物污染风险的限制。在本研究中，对与细菌共培养的微藻的生理生化参数进行了研究。先前的一项研究报道了微藻与细菌共培养的益处；然而，并未进一步研究细菌生长及分子机制。我们的结果表明，与不与细菌共培养的纯培养相比，这种共培养使微藻的总叶绿素、生长、干重和储存糖副淀粉含量均显著增加。该分析是对微藻 - 细菌共培养的首次全面代谢组学研究，共鉴定出339种代谢物。结果表明，该共培养系统在微藻细胞和细菌细胞之间具有协同代谢相互作用，与微藻单独培养相比，氨基甲酸甲酯、四氢嘧啶、胆碱、N - 甲基邻氨基苯甲酸甲酯、龙胆酸二甲酯、4R - 氨基戊酸和谷氨酰胺 - 缬氨酸显著增加。综上所述，这些结果填补了当前对微藻 - 细菌共培养系统理解上的重大空白，并为微藻大规模生产和工业应用的潜在改进提供了新的见解。
