Ministry of Education, Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Chongqing, China.
Chongqing Science and Technology Bureau, Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Chongqing, China.
Luminescence. 2021 Aug;36(5):1317-1326. doi: 10.1002/bio.4059. Epub 2021 May 24.
As a natural enzyme, alkaline phosphatase (ALP) plays an essential role in clinicopathological examinations and biomedical research, and is capable of hydrolyzing the phosphate group of l-ascorbic acid-2-phosphate (AAP) to yield l-ascorbic acid (L-AA). L-AA reduced cobalt oxyhydroxide (CoOOH) nanoflakes to Co , leading to a smaller size and weaker light scattering, which could be monitored by electron microscopic images and optical spectra. The indirect detection of ALP was achieved by the reduced light scattering signal of CoOOH nanoflakes. Under optimal conditions, the decrease in scattering intensity was proportional to the ALP concentration over the range 0.1-160 U/L and the detection limit was 0.034 U/L (3σ/k). Compared with other assays, this proposed light scattering method was more convenient and economic for ALP sensing. The method was successfully applied to ALP analysis in human serum samples, and was similar to the results obtained by commercial kits.
作为一种天然酶，碱性磷酸酶（ALP）在临床病理检查和生物医学研究中发挥着重要作用，能够水解 l-抗坏血酸-2-磷酸（AAP）的磷酸基团，生成 l-抗坏血酸（L-AA）。L-AA 将氧化钴羟氢（CoOOH）纳米片还原为 Co，导致尺寸更小且光散射减弱，可以通过电子显微镜图像和光谱进行监测。通过 CoOOH 纳米片光散射信号的降低实现了对 ALP 的间接检测。在最佳条件下，散射强度的降低与 0.1-160 U/L 范围内的 ALP 浓度成正比，检测限为 0.034 U/L（3σ/k）。与其他测定方法相比，这种光散射方法在 ALP 感测方面更加方便和经济。该方法成功应用于人血清样品中的 ALP 分析，与商业试剂盒的结果相似。
