Metabiota Cameroon Ltd, Yaoundé, Centre Region, Cameroon.
Faculty of Medicine and Biomedical Sciences, University of Yaoundé, Yaoundé, Centre Region, Cameroon.
Microb Genom. 2021 Apr;7(4). doi: 10.1099/mgen.0.000561.
Adenoviruses (AdVs) are diverse pathogens of humans and animals, with several dozen bat AdVs already identified. Considering that over 100 human AdVs are known, and the huge diversity of bat species, many bat AdVs likely remain undiscovered. To learn more about AdV prevalence, diversity and evolution, we sampled and tested bats in Cameroon using several PCR assays for viral and host DNA. AdV DNA was detected in 14 % of the 671 sampled animals belonging to 37 different bat species. There was a correlation between species roosting in larger groups and AdV DNA detection. The detected AdV DNA belonged to between 28 and 44 different, mostly previously unknown, mastadenovirus species. The novel isolates are phylogenetically diverse and while some cluster with known viruses, others appear to form divergent new clusters. The phylogenetic tree of novel and previously known bat AdVs does not mirror that of the various host species, but does contain structures consistent with a degree of virus-host co-evolution. Given that closely related isolates were found in different host species, it seems likely that at least some bat AdVs have jumped species barriers, probably in the more recent past; however, the tree is also consistent with such events having taken place throughout bat AdV evolution. AdV diversity was highest in bat species roosting in large groups. The study significantly increased the diversity of AdVs known to be harboured by bats, and suggests that host behaviours, such as roosting size, may be what limits some AdVs to one species rather than an inability of AdVs to infect other related hosts.
腺病毒（AdV）是人类和动物的多种病原体，已有数十种蝙蝠 AdV 被鉴定。考虑到已知有超过 100 种人类 AdV，以及蝙蝠种类的巨大多样性，许多蝙蝠 AdV 可能尚未被发现。为了更多地了解 AdV 的流行率、多样性和进化，我们使用几种针对病毒和宿主 DNA 的 PCR 检测方法在喀麦隆对蝙蝠进行了采样和检测。在所检测的 671 只来自 37 种不同蝙蝠物种的动物中，有 14%检测到了 AdV DNA。在群体较大的蝙蝠物种中，与 AdV DNA 检测存在相关性。检测到的 AdV DNA 属于 28 到 44 种不同的、主要是以前未知的巨细胞病毒物种。新分离株具有多样化的系统发育，虽然有些与已知病毒聚类，但有些似乎形成了不同的新聚类。新型和以前已知的蝙蝠 AdV 的系统发育树与各种宿主物种的系统发育树并不一致，但确实包含了与病毒-宿主共同进化程度一致的结构。由于在不同的宿主物种中发现了密切相关的分离株，因此至少有一些蝙蝠 AdV 很可能在最近的过去跨越了物种障碍；然而，该树也与 AdV 进化过程中发生此类事件的情况一致。在群体较大的蝙蝠物种中，AdV 的多样性最高。该研究显著增加了已知由蝙蝠携带的 AdV 多样性，并表明宿主行为，如群体大小，可能是限制某些 AdV 仅在一个物种中存在而不是 AdV 无法感染其他相关宿主的原因。
