Posgrado en Ciencias Biológicas, Instituto de Biología, Universidad Nacional Autónoma de México, s/n, Ciudad Universitaria, Coyoacán, Ciudad de México, C.P. 04510, Mexico.
Instituto de Biología, Universidad Nacional Autónoma de México, Tercer circuito exterior s/n, Ciudad Universitaria, Coyoacán, Ciudad de México, C.P. 04510, Mexico.
Parasitol Int. 2021 Aug;83:102352. doi: 10.1016/j.parint.2021.102352. Epub 2021 Apr 16.
Species in the genus Gorgoderina Looss, 1902 are parasites of the urinary bladder of amphibians and include around 50 species described globally. Molecular data on species of the genus are scarce, as is the information of their life-cycle patterns. During a survey on the genetic characterization of the frog trematodes in the tropical rain forest of Los Tuxtlas, in the Gulf of Mexico slope of Mexico, specimens of two morphotypes of Gorgoderina were sampled from the Rio Grande leopard frog, Rana berlandieri. One of them represented an undescribed species which is described herein as Gorgoderina rosamondae n. sp., whereas the other one was morphologically very similar to an apparently widely distributed North American species, G. attenuata, which has been previously reported in the same geographical area. Specimens of both morphotypes were sequenced for two nuclear and one mitochondrial genes. Phylogenetic trees corroborated the distinction of the new species, and data on the internal transcribed spacer 2 revealed genetic differences between G. attenuata sequenced from frogs in USA and specimens of Gorgoderina sp. from Los Tuxtlas, indicating the possibility that they also represent an undescribed species. COI sequences showed high genetic divergence values between the new species and Gorgoderina sp. from Los Tuxtlas (8.63-9.99%). Additionally, COI sequences of the larval forms (sporocyst, cercariae and metacercariae) sampled in the same locality from their first and second intermediate hosts (Pisidium sp. and Agriogomphus tumens, respectively) showed conspecificity, and the 3 host life-cycle of the new species was elucidated.
物种在属 Gorgoderina Looss，1902 是寄生虫的膀胱两栖类动物，包括约 50 种描述全球。分子数据的物种属是稀缺的，因为是他们的生命周期模式的信息。在调查的遗传特征的青蛙吸虫在热带雨林的洛斯图克斯拉斯，在墨西哥湾的斜坡，墨西哥，标本的两种形态的 Gorgoderina 被采样从里奥格兰德豹蛙，Rana berlandieri。其中一个代表一个未描述的物种是本文描述的 Gorgoderina rosamondae n. sp.，而另一个形态非常相似的明显广泛分布的北美物种，G. attenuata，这是以前报道在同一地理区域。标本的两种形态进行了测序的两个核和一个线粒体基因。系统发育树证实了新物种的区别，和数据的内部转录间隔 2 显示遗传差异 betweenG. attenuata 测序从青蛙在美国和标本的 Gorgoderina sp. 从洛斯图克斯拉斯，表明他们也可能代表一个未描述的物种。COI 序列显示高遗传分化值之间的新物种和 Gorgoderina sp. 从洛斯图克斯拉斯（8.63-9.99%）。此外，COI 序列的幼虫形式（孢子囊，尾蚴和囊蚴）采样在同一地点从他们的第一和第二中间宿主（Pisidium sp. 和 Agriogomphus tumens，分别）表现出同种，和 3 新物种的生活周期宿主阐明。
