工业级聚乙烯和聚丙烯微塑料不同粒径范围对赤子爱胜蚓的生态毒理学效应。
Ecotoxicological effects of different size ranges of industrial-grade polyethylene and polypropylene microplastics on earthworms Eisenia fetida.
机构信息
Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, China.
Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, China.
出版信息
Sci Total Environ. 2021 Aug 20;783:147007. doi: 10.1016/j.scitotenv.2021.147007. Epub 2021 Apr 10.
The effects of microplastics (MPs) on terrestrial organisms remain poorly understood, even though soil is an important MPs sink. In this study, the earthworms Eisenia fetida were exposed to 0.25% (w/w) of industrial-grade high-density polyethylene (HDPE, 28-145, 133-415 and 400-1464 μm) and polypropylene (PP, 8-125, 71-383 and 761-1660 μm) MPs in an agricultural soil for 28 d. The results showed that HDPE and PP MPs with different size ranges can be ingested by E. fetida. Exposure to different size ranges of HDPE and PP MPs altered the activities of superoxide dismutase, catalase and glutathione S-transferase and induced an increase in the 8-hydroxy-2'-deoxyguanosine level in E. fetida, suggesting that MPs-induced oxidative stress occurred in E. fetida. A size and type-dependent toxicity of MPs to E. fetida was demonstrated by the integrated biological response index. In addition, to obtain detailed molecular information on the responses of E. fetida to MPs exposure, transcriptomic analysis was conducted for E. fetida from HDPE (28-145 μm) and PP (8-125 μm) treatment groups. Transcriptomic analysis identified 34,937 and 28,494 differentially expressed genes in the HDPE and PP MPs treatments compared with the control, respectively. And, exposure to HDPE and PP MPs significantly disturbed several pathways closely related to neurodegeneration, oxidative stress and inflammatory responses in E. fetida. This study provides important information for the ecological risk assessment of different size ranges and types of industrial-grade MPs.
微塑料（MPs）对陆地生物的影响仍知之甚少，尽管土壤是 MPs 的重要汇。在这项研究中，赤子爱胜蚓（Eisenia fetida）被暴露于农业土壤中 0.25%（w/w）的工业级高密度聚乙烯（HDPE，28-145、133-415 和 400-1464μm）和聚丙烯（PP，8-125、71-383 和 761-1660μm） MPs 中 28 天。结果表明，不同大小范围的 HDPE 和 PP MPs 可被赤子爱胜蚓（Eisenia fetida）摄入。暴露于不同大小范围的 HDPE 和 PP MPs 改变了超氧化物歧化酶、过氧化氢酶和谷胱甘肽 S-转移酶的活性，并诱导赤子爱胜蚓（Eisenia fetida）中 8-羟基-2'-脱氧鸟苷水平升高，表明 MPs 诱导的氧化应激发生在赤子爱胜蚓（Eisenia fetida）中。综合生物反应指数表明 MPs 对赤子爱胜蚓（Eisenia fetida）具有大小和类型依赖性毒性。此外，为了获得赤子爱胜蚓（Eisenia fetida）对 MPs 暴露反应的详细分子信息，对赤子爱胜蚓（Eisenia fetida）进行了 HDPE（28-145μm）和 PP（8-125μm）处理组的转录组分析。转录组分析分别在 HDPE 和 PP MPs 处理组与对照组之间鉴定了 34937 个和 28494 个差异表达基因。并且，暴露于 HDPE 和 PP MPs 显著扰乱了赤子爱胜蚓（Eisenia fetida）中几个与神经退行性变、氧化应激和炎症反应密切相关的途径。这项研究为不同大小范围和类型的工业级 MPs 的生态风险评估提供了重要信息。
Zotero 插件