Institute of Biomolecular Chemistry, National Research Council, Via Campi Flegrei 34, 80078 Pozzuoli, Napoli, Italy; Department of Biology, Faculty of Science, Hong Kong Baptist University, Kowloon Tong, Hong Kong Special Administrative Region.
Institute of Biomolecular Chemistry, National Research Council, Via Campi Flegrei 34, 80078 Pozzuoli, Napoli, Italy.
Bioresour Technol. 2021 Jul;332:125127. doi: 10.1016/j.biortech.2021.125127. Epub 2021 Apr 6.
This study investigated the feasibility of hydrogen (H) and L-lactic acid production from starch under capnophilic lactic fermentation (CLF) conditions by using Thermotoga neapolitana. Batch experiments were performed in 120 mL serum bottles and a 3 L pH-controlled continuous stirred-tank reactors (CSTR) system with potato and wheat starch as the substrates. A H yield of 3.34 (±0.17) and 2.79 (±0.17) mol H/mol of glucose eq. was achieved with, respectively, potato and wheat starch. In the presence of CO, L-lactic acid production by the acetyl-CoA carboxylation was significantly higher for the potato starch (0.88 ± 0.39 mol lactic acid/mol glucose eq.) than wheat starch (0.33 ± 0.11 mol lactic acid/mol glucose eq.). A kinetic model was applied to simulate and predict the T. neapolitana metabolic profile and bioreactor performance under CLF conditions. The CLF-based starch fermentation suggests a new direction to biotransform agri-food waste into biofuels and valuable biochemicals.
本研究利用嗜热栖热菌（Thermotoga neapolitana），考察了在嗜二氧化碳乳酸发酵（Capnophilic lactic fermentation，CLF）条件下，从淀粉生产氢气（H）和 L-乳酸的可行性。在 120 毫升血清瓶和 3 L pH 控制连续搅拌釜式反应器（Continuous stirred-tank reactors，CSTR）系统中进行分批实验，以马铃薯淀粉和小麦淀粉为底物。分别用马铃薯淀粉和小麦淀粉作为底物，实现了 3.34（±0.17）和 2.79（±0.17）mol H/mol 葡萄糖当量的 H 产率。在 CO 的存在下，通过乙酰辅酶 A 羧化作用生产 L-乳酸，马铃薯淀粉（0.88±0.39 mol 乳酸/mol 葡萄糖当量）的产量明显高于小麦淀粉（0.33±0.11 mol 乳酸/mol 葡萄糖当量）。应用动力学模型模拟和预测了嗜二氧化碳乳酸发酵条件下 T. neapolitana 的代谢特征和生物反应器性能。基于 CLF 的淀粉发酵为将农业食品废物转化为生物燃料和有价值的生化物质提供了一个新的方向。
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