Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, Via Di Biasio, 43, 03043 Cassino, FR, Italy.
Department of Civil, Architectural and Environmental Engineering, University of Napoli Federico II, Via Claudio 21, 80125 Napoli, Italy.
Bioresour Technol. 2019 Dec;293:122033. doi: 10.1016/j.biortech.2019.122033. Epub 2019 Aug 18.
This study focused on continuous-flow hydrogen production by Thermotoga neapolitana at a hydraulic retention time (HRT) decreasing from 24 to 5 h. At each HRT reduction, the hydrogen yield (HY) immediately dropped, but recovered during prolonged cultivation at constant HRT. The final HY in each operating period decreased from 3.4 (±0.1) to 2.0 (±0.0) mol H/mol glucose when reducing the HRT from 24 to 7 h. Simultaneously, the hydrogen production rate (HPR) and the liquid phase hydrogen concentration (H) increased from 82 (±1) to 192 (±4) mL/L/h and from 9.1 (±0.3) to 15.6 (±0.7) mL/L, respectively. Additionally, the effluent glucose concentration increased from 2.1 (±0.1) to above 10 mM. Recirculating H-rich biogas prevented the supersaturation of H reaching a value of 9.3 (±0.7) mL/L, resulting in complete glucose consumption and the highest HPR of 277 mL/L/h at an HRT of 5 h.
本研究关注的是在水力停留时间（HRT）从 24 小时减少到 5 小时的情况下，嗜热栖热菌连续产氢。在每次 HRT 降低时，产氢率（HY）立即下降，但在恒定 HRT 下长时间培养时会恢复。当 HRT 从 24 小时减少到 7 小时时，每个运行阶段的最终 HY 从 3.4（±0.1）降低到 2.0（±0.0）摩尔 H/摩尔葡萄糖。同时，氢气产率（HPR）和液相氢气浓度（H）分别从 82（±1）增加到 192（±4）mL/L/h 和从 9.1（±0.3）增加到 15.6（±0.7）mL/L。此外，流出物葡萄糖浓度从 2.1（±0.1）增加到 10 mM 以上。循环富氢沼气防止了氢气达到 9.3（±0.7）mL/L 的过饱和状态，导致葡萄糖完全消耗，并在 HRT 为 5 小时时达到最高的 HPR 为 277 mL/L/h。
