Rajapaksha Asanka Amith, Fu Yi-Xuan, Guo Wu Yingzheng, Liu Shi-Yu, Li Zhi-Wen, Xiong Cui-Qin, Yang Wen-Chao, Yang Guang-Fu
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, People's Republic of China.
Department of Nano Science Technology, Faculty of Technology, Wayamba University of Sri Lanka, Kuliyapitiya, Sri Lanka.
Methods Appl Fluoresc. 2021 Apr 30;9(3). doi: 10.1088/2050-6120/abf988.
Enzymes are very important for biological processes in a living being, performing similar or multiple tasks in and out of cells, tissues and other organisms at a particular location. The abnormal activity of particular enzyme usually caused serious diseases such as Alzheimer's disease, Parkinson's disease, cancers, diabetes, cardiovascular diseases, arthritis etc. Hence, nondestructive and real-time visualization for certain enzyme is very important for understanding the biological issues, as well as the drug administration and drug metabolism. Fluorescent cellular probe-based enzyme detectionandhas become broad interest for human disease diagnostics and therapeutics. This review highlights the recent findings and designs of highly sensitive and selective fluorescent cellular probes targeting enzymes for quantitative analysis and bioimaging.
酶对于生物体内的生物过程非常重要，在细胞、组织和其他生物体的特定位置内外执行相似或多种任务。特定酶的异常活性通常会引发严重疾病，如阿尔茨海默病、帕金森病、癌症、糖尿病、心血管疾病、关节炎等。因此，对特定酶进行无损且实时的可视化对于理解生物学问题以及药物给药和药物代谢非常重要。基于荧光细胞探针的酶检测已在人类疾病诊断和治疗方面引起了广泛关注。本文综述重点介绍了针对酶的高灵敏度和高选择性荧光细胞探针的最新研究成果与设计，用于定量分析和生物成像。
