Russell Angela J, Bidartondo Martin I, Butterfield Brian G
Department of Plant and Microbial Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand.
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3120, USA.
New Phytol. 2002 Nov;156(2):283-295. doi: 10.1046/j.1469-8137.2002.00504.x.
• Here we present the ultrastructure and molecular identification of the fungi in the mycorrhizal nodules of four species of Podocarpaceae endemic to New Zealand. Podocarps form a major component of the rain forest of New Zealand, where they grow on soils poor in extractable phosphate and high in organic matter. • Histological studies showed that the mycorrhizal nodules develop as modified lateral roots, and are colonised by an endophytic fungus. Scanning and transmission electron microscopy showed the fungus to contain intracellular coils and arbuscules typical of the order Glomales. • DNA was extracted and amplified using PCR to identify mycorrhizal fungi. Several different lineages of arbuscular mycorrhizal fungi colonising the nodules were identified. Individual nodules contained more than one fungal lineage. • This study is the first to indicate species of Glomales present in the New Zealand rain forest. It is likely that many of the taxa sampled are new to science because there has been little taxonomic work on Australasian Glomales.
• 在此，我们展示了新西兰特有的四种罗汉松科植物菌根瘤中真菌的超微结构和分子鉴定。罗汉松是新西兰雨林的主要组成部分，它们生长在可提取磷含量低、有机质含量高的土壤上。
• 组织学研究表明，菌根瘤是由侧根变异发育而成，并被一种内生真菌定殖。扫描电子显微镜和透射电子显微镜显示，该真菌含有球囊霉目典型的细胞内线圈和丛枝。
• 提取DNA并使用PCR进行扩增，以鉴定菌根真菌。鉴定出了几种定殖在瘤中的不同谱系的丛枝菌根真菌。单个瘤中含有不止一种真菌谱系。
• 这项研究首次表明新西兰雨林中存在球囊霉目物种。很可能所采样的许多分类单元对科学界来说都是新的，因为对澳大拉西亚球囊霉目的分类学研究很少。
