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对蒺藜苜蓿体外根培养物进行靶向接种,揭示了丛枝菌根真菌感染早期阶段蒺藜苜蓿 MtENOD11 的表达情况。

Targeted inoculation of Medicago truncatula in vitro root cultures reveals MtENOD11 expression during early stages of infection by arbuscular mycorrhizal fungi.

作者信息

Chabaud M, Venard C, Defaux-Petras A, Bécard G, Barker D G

机构信息

Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, UMR215 INRA-CNRS, BP 27, 31326 Castanet Tolosan Cedex, France.

Signaux et Messages Cellulaires Chez les Végétaux, UMR 5546 CNRS-Université P. Sabatier, Pôle de Biotechnologie Végétale, BP 17, 31326 Castanet Tolosan Cedex, France.

出版信息

New Phytol. 2002 Nov;156(2):265-273. doi: 10.1046/j.1469-8137.2002.00508.x.

Abstract

•  An in vitro targeted inoculation technique has been developed for studying the earliest stages of arbuscular endomycorrhizal (AM) infection of Medicago truncatula roots, and in particular the spatio-temporal expression of the early nodulin gene MtENOD11. •  Agrobacterium rhizogenes transformed root explants were derived either from Myc M. truncatula or from the infection-defective Myc mutant TR26 ( dmi2-2 ), both expressing the pMtENOD11-gusA fusion. The normal positive geotropism of these roots, coupled with the negative geotropism of Gigaspora germ tubes allowed oriented growth of the two symbiotic partners, facilitating the identification of initial fungal/root contacts. •  Early infection events at the stage of appressoria and/or internal hyphae could be observed for over 50% of the inoculated explants, revealing that MtENOD11 is expressed transiently in both epidermal and cortical cells at sites of hyphal penetration in Myc roots, but not in epidermal cells in contact with appressoria in Myc roots. •  We propose that a direct link exists between MtENOD11 gene expression and cellular events required for fungal penetration, thereby extending analogies between rhizobial and AM host root infection processes.

摘要

• 已开发出一种体外靶向接种技术,用于研究蒺藜苜蓿根丛枝内生菌根(AM)感染的早期阶段,特别是早期结瘤素基因MtENOD11的时空表达。• 发根农杆菌转化的根外植体来源于蒺藜苜蓿野生型或感染缺陷型突变体TR26(dmi2-2),二者均表达pMtENOD11-gusA融合基因。这些根的正常正向地性,加上巨孢囊霉孢子管的负向地性,使得两个共生伙伴能够定向生长,便于识别最初的真菌/根接触点。• 对于超过50%的接种外植体,可以观察到附着胞和/或内部菌丝阶段的早期感染事件,这表明MtENOD11在野生型根中菌丝穿透部位的表皮和皮层细胞中短暂表达,但在与附着胞接触的突变体根的表皮细胞中不表达。• 我们认为MtENOD11基因表达与真菌穿透所需的细胞事件之间存在直接联系,从而扩展了根瘤菌与AM宿主根感染过程之间的相似性。

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