College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Chinese Fir Engineering Technology Research Center of the State Forestry and Grassland Administration, Fuzhou, Fujian, China.
PeerJ. 2024 Mar 22;12:e17138. doi: 10.7717/peerj.17138. eCollection 2024.
The continuous establishment of Chinese fir () plantations across multiple generations has led to the limited impact of soil phosphorus (P) on tree growth. This challenge poses a significant obstacle in maintaining the sustainable management of Chinese fir.
To investigate the effects of Arbuscular mycorrhizal fungi (AMF) on the growth and physiological characteristics of Chinese fir under different P supply treatments. We conducted an indoor pot simulation experiment in the greenhouse of the Forestry College of Fujian Agriculture and Forestry University with one-and-half-year-old seedlings of Chinese fir from March 2019 to June 2019, with the two P level treatment groups included a normal P supply treatment (1.0 mmol L KHPO, P1) and a no P supply treatment (0 mmol L KHPO, P0). P0 and P1 were inoculated with () or () or not inoculated with AMF treatment. The AMF colonization rate in the root system, seedling height (SH), root collar diameter (RCD) growth, chlorophyll (Chl) photosynthetic characteristics, enzyme activities, and endogenous hormone contents of Chinese fir were estimated.
The results showed that the colonization rate of in the roots of Chinese fir seedlings was the highest at P0, up to 85.14%, which was 1.66 times that of P1. Under P0 and P1 treatment, root inoculation with either or promoted SH growth, the SH of treatment was 1.38 times and 1.05 times that of treatment, respectively. In the P1 treatment, root inoculation with either or inhibited RCD growth. inhibited RCD growth more aggressively than . In the P0 treatment, root inoculation with and reduced the inhibitory effect of phosphorus deficiency on RCD. At this time, there was no significant difference in RCD between , and treatments ( < 0.05). AMF inoculation increased , , , and during the chlorophyll fluorescence response in the tested Chinese fir seedlings. Under the two phosphorus supply levels, the trend of and of Chinese fir seedlings in different treatment groups was > > CK. Under P0 treatment, The values of were 235.86, 221.86 and 147.71, respectively. The values of were 287.57, 275.71 and 201.57, respectively. It increased the antioxidant enzyme activity and reduced the leaf's malondialdehyde (MDA) content to a certain extent.
It is concluded that AMF can enhance the photosynthetic capacity of the host, regulate the distribution of endogenous hormones in plants, and promote plant growth by increasing the activity of antioxidant enzymes. When the P supply is insufficient, AMF is more helpful to plants, and is more effective than in alleviating P starvation stress in Chinese fir.
多代营建的杉木（）人工林导致土壤磷（P）对树木生长的影响有限。这一挑战对维持杉木的可持续管理构成了重大障碍。
为了研究丛枝菌根真菌（AMF）对不同磷供应处理下杉木生长和生理特性的影响。我们于 2019 年 3 月至 6 月在福建农林大学林学院温室中进行了一项室内盆栽模拟实验，使用 1 年半大小的杉木幼苗，有两个磷水平处理组，包括正常磷供应处理（1.0 mmol L KHPO，P1）和无磷供应处理（0 mmol L KHPO，P0）。P0 和 P1 接种（）或（）或不接种 AMF 处理。估计了 AMF 在根系中的定殖率、苗高（SH）、根颈直径（RCD）生长、叶绿素（Chl）光合作用特性、酶活性和杉木内源激素含量。
结果表明，在 P0 条件下，杉木幼苗根系中 的定殖率最高，达到 85.14%，是 P1 的 1.66 倍。在 P0 和 P1 处理下，根接种或均促进了 SH 的生长，处理的 SH 分别是处理的 1.38 倍和 1.05 倍。在 P1 处理下，根接种或均抑制了 RCD 的生长。比更能抑制 RCD 的生长。在 P0 处理下，根接种和减少了磷缺乏对 RCD 的抑制作用。此时，、和处理之间的 RCD 没有显著差异（<0.05）。AMF 接种增加了在受测杉木幼苗的叶绿素荧光响应过程中的、、、。在两种供磷水平下，不同处理组杉木幼苗的和趋势为> > CK。在 P0 处理下，的值分别为 235.86、221.86 和 147.71，的值分别为 287.57、275.71 和 201.57。它在一定程度上提高了抗氧化酶的活性，降低了叶片丙二醛（MDA）的含量。
丛枝菌根真菌可以通过提高宿主的光合作用能力、调节植物内源激素的分布、提高抗氧化酶的活性来促进植物的生长。当磷供应不足时，AMF 对植物更有帮助，在缓解杉木磷饥饿胁迫方面比更有效。
