Hanikenne M
Genetics of Microorganisms, Department of Life Sciences, B22, University of Liège, B4000 Liège, Belgium.
New Phytol. 2003 Aug;159(2):331-340. doi: 10.1046/j.1469-8137.2003.00788.x.
The green alga Chlamydomonas reinhardtii is a useful model of a photosynthetic cell. This unicellular eukaryote has been intensively used for studies of a number of physiological processes such as photosynthesis, respiration, nitrogen assimilation, flagella motility and basal body function. Its easy-to-manipulate and short life cycle make this organism a powerful tool for genetic analysis. Over the past 15 yr, a dramatically increased number of molecular technologies (including nuclear and organellar transformation systems, cosmid, yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) libraries, reporter genes, RNA interference, DNA microarrays, etc.) have been applied to Chlamydomonas. Moreover, as parts of the Chlamydomonas genome project, molecular mapping, as well as whole genome and extended expressed sequence tag (EST) sequencing programs, are currently underway. These developments have allowed Chlamydomonas to become an extremely valuable model for molecular approaches to heavy metal homeostasis and tolerance in photosynthetic organisms.
莱茵衣藻这种绿藻是光合细胞的一种有用模型。这种单细胞真核生物已被广泛用于研究许多生理过程，如光合作用、呼吸作用、氮同化作用、鞭毛运动和基体功能。其易于操作的特性和较短的生命周期使该生物体成为遗传分析的有力工具。在过去15年中，大量分子技术（包括核转化和细胞器转化系统、黏粒、酵母人工染色体（YAC）和细菌人工染色体（BAC）文库、报告基因、RNA干扰、DNA微阵列等）已应用于衣藻研究。此外，作为衣藻基因组计划的一部分，分子图谱绘制以及全基因组和扩展表达序列标签（EST）测序项目目前正在进行。这些进展使衣藻成为研究光合生物中重金属稳态和耐受性分子方法的极有价值的模型。
