Chen David M, Bastias Brigitte A, Taylor Andrew F S, Cairney John W G
Mycorrhiza Research Group, Centre for Horticulture and Plant Sciences, Parramatta Campus, University of Western Sydney, Locked Bag 1797, Penrith South and NSW 1797, Australia.
Department of Forest Mycology & Pathology, Swedish University of Agricultural Sciences, Box 7026, S-75007 Uppsala, Sweden.
New Phytol. 2003 Mar;157(3):547-554. doi: 10.1046/j.1469-8137.2003.00687.x.
• Ectomycorrhizal (ECM) fungi were screened for laccase-like genes by polymerase chain reaction (PCR) using primers for white rot fungal laccase genes, and expression of the genes was examined by reverse transcriptase polymerase chain reaction (RT-PCR) for Piloderma byssinum in axenic culture under different nutrient conditions. • Laccase-like genes were present in Rhizopogon roseolus along with several Russulales and Atheliaceae taxa, and showed strong nucleotide sequence similarity to laccase genes in white rot fungi. Multiple laccase-like genes were only identified in Piloderma spp. • Laccase-like genes were expressed in Piloderma spp., with transcript levels some six times higher under high nitrogen conditions in P. byssinum than when nitrogen availability was lower. • The potential roles of laccases in nutrient mobilization and/or differentiation of multihyphal ECM fungal structures are discussed.
• 使用白腐真菌漆酶基因引物，通过聚合酶链反应（PCR）筛选外生菌根（ECM）真菌中的漆酶样基因，并在不同营养条件下，通过逆转录聚合酶链反应（RT-PCR）检测无菌培养的棉状皮壳菌（Piloderma byssinum）中这些基因的表达情况。
• 玫瑰红根须腹菌（Rhizopogon roseolus）以及几个红菇目（Russulales）和阿太菌科（Atheliaceae）分类群中存在漆酶样基因，并且与白腐真菌中的漆酶基因具有很强的核苷酸序列相似性。仅在皮壳菌属（Piloderma spp.）中鉴定出多个漆酶样基因。
• 漆酶样基因在皮壳菌属中表达，在高氮条件下，棉状皮壳菌中的转录水平比氮供应较低时高约六倍。
• 讨论了漆酶在多菌丝外生菌根真菌结构的养分调动和/或分化中的潜在作用。
