Panteris Emmanuel, Apostolakos Panagiotis, Quader Hartmut, Galatis Basil
University of Athens, Faculty of Biology, Department of Botany, Athens 15784, Greece.
University of Hamburg, Institute of General Botany, D-22609 Hamburg, Germany.
New Phytol. 2004 Aug;163(2):271-286. doi: 10.1111/j.1469-8137.2004.01125.x.
• The role of actomyosin and microtubules (MTs) in division plane establishment and cell plate expansion was studied in vacuolated cells of Coleus. • Fluorescence and conventional microscopy were applied on control material and after treatment with anti-MT and anti-actin filament (AF) drugs as well as myosin inhibitors. • The division plane is predicted by a cortical cytoplasmic ring (CCR), rich in AFs and endoplasmic reticulum, formed at interphase. The nucleus migrates to the CCR before its entrance in the phragmosome. The latter consists of transvacuolar cytoplasmic strands connecting the perinuclear cytoplasm with the CCR. During preprophase, a preprophase MT band is organized in the CCR. AF disruption and myosin inhibition destroy the CCR and cytoplasmic strands, arrest migration of the nucleus and affect cell plate expansion. MT disorganization inhibits anchoring of the cytoplasmic strands into the division plane. • These observations support that CCR establishment, formation of transvacuolar cytoplasmic strands, migration of the nucleus and lateral cell plate expansion depend on actomyosin. The MTs guide and anchor the cytoplasmic strands of the phragmosome into the predetermined division plane.
• 研究了肌动球蛋白和微管（MTs）在彩叶草液泡化细胞的分裂平面建立和细胞板扩展中的作用。
• 对对照材料以及用抗MT、抗肌动蛋白丝（AF）药物和肌球蛋白抑制剂处理后的材料进行了荧光显微镜和传统显微镜观察。
• 分裂平面由一个富含AFs和内质网的皮质细胞质环（CCR）预测，该环在间期形成。细胞核在进入成膜体之前迁移到CCR。成膜体由连接核周细胞质和CCR的跨液泡细胞质丝组成。在前期，一条前期MT带在CCR中形成。AF破坏和肌球蛋白抑制会破坏CCR和细胞质丝，阻止细胞核迁移并影响细胞板扩展。MT紊乱会抑制细胞质丝锚定到分裂平面。
• 这些观察结果支持CCR的建立、跨液泡细胞质丝的形成、细胞核的迁移和细胞板的横向扩展依赖于肌动球蛋白。MTs引导并将成膜体的细胞质丝锚定到预定的分裂平面。
