Williams Michael L, Cobb A H
Department of Life Sciences, Trent Polytechnic, Burton Street, Nottingham NG1 4BU, UK.
New Phytol. 1985 Sep;101(1):79-88. doi: 10.1111/j.1469-8137.1985.tb02817.x.
Net starch synthesis by isolated chloroplasts of the intertidal alga Codium fragile (Suringar) Hariot was determined by enzymic hydrolysis (amyloglucosidase/α-amylase) of solubilized chloroplast samples followed by the measurement of the resultant glucose yields by hexokinase/glucose-6-phosphate dehydrogenase-coupled reactions. Net starch synthesis was only observed between 100 and 375 μmol m s (PAR) corresponding to irradiance values which supported saturating or super-saturating rates of photosynthesis. Light filter inactivation of the light-harvesting complex carotenoids siphonoxanthin and siphonein reduced net starch synthesis by 68 %. These findings are discussed in relation to photosynthesis in an intertidal zone environment.
通过对潮间带藻类柔弱刚毛藻（Suringar）Hariot分离的叶绿体进行净淀粉合成测定，方法是对溶解的叶绿体样品进行酶促水解（淀粉葡糖苷酶/α-淀粉酶），然后通过己糖激酶/葡萄糖-6-磷酸脱氢酶偶联反应测量所得葡萄糖产量。仅在100至375 μmol m s（光合有效辐射）之间观察到净淀粉合成，这对应于支持光合作用饱和或超饱和速率的辐照度值。捕光复合物类胡萝卜素虹吸叶黄素和管藻素的滤光失活使净淀粉合成减少了68%。结合潮间带环境中的光合作用对这些发现进行了讨论。
