Watanabe I, Lin Chang, Santiago-Ventura Teresita
Department of Soil Microbiology, The International Rice Research Institute, P.O. Box 933, Manila, Philippines.
New Phytol. 1989 Apr;111(4):625-630. doi: 10.1111/j.1469-8137.1989.tb02356.x.
The responses to high temperature of the cyanobacterial symbiont and the host fern in tile Azolla-Anabaena symbiosis were studied by using Azolla strains with heterologous Anabaena azollae Strasburger of without Anabaena. At 26/18°C, symbiotic Azolla grew better than the corresponding Anabaena-free Azolla. A high temperature-sensitive species Azolla filiculoides Lamarck, produced smaller biomass under conditions of 37 °C (day)/29 °C (night) than the corresponding Anabaena-free Azolla grown in N-containing medium. A. filiculoides with Anabaena from temperature tolerant Azolla microphylla Kaulfuss was more effective at N fixation than A. filiculoides with homologous Anabaena. A microphylla with Anabaena from A. filiculoides grew more slowly and fixed less N than the strains with homologous Anabaena. In the presence of combined N at 37°/29 °C, A. microphylla grew better than A. filiculoides with Anabaena from A. microphylla. Tolerance of the Azolla-Anabaena association to high temperature is, therefore, determined both in the host fern and symbiont Anabaena.
通过使用含有异源满江红鱼腥藻斯特拉斯堡种或不含鱼腥藻的满江红菌株，研究了满江红-鱼腥藻共生体系中蓝藻共生体和宿主蕨对高温的响应。在26/18°C条件下，共生满江红比相应的无菌满江红生长得更好。高温敏感物种细叶满江红在37°C（白天）/29°C（夜晚）条件下产生的生物量比在含氮培养基中生长的相应无菌满江红小。含有来自耐温小叶满江红的鱼腥藻的细叶满江红在固氮方面比含有同源鱼腥藻的细叶满江红更有效。含有来自细叶满江红的鱼腥藻的小叶满江红比含有同源鱼腥藻的菌株生长更慢且固氮更少。在37°/29°C且存在化合态氮的情况下，小叶满江红比含有来自小叶满江红的鱼腥藻的细叶满江红生长得更好。因此，满江红-鱼腥藻共生体对高温的耐受性由宿主蕨和共生体鱼腥藻共同决定。
